Egr-IFNγ基因治疗联合^125Ⅰ-脱氧尿嘧啶核苷治疗抑瘤效应的实验研究  

Anti-tumor effects of Egr-IFNγ gene therapy combined with ^125Ⅰ-UdR radionucfide therapy

作　　者：赵敬国 倪彦君[2] 孙婷[5] 宋享福[3] 马庆杰[6] 李修义[3] 高凤彤[6] 杨巍[4] 

机构地区：[1]解放军第四0三医院,大连116015 [2]沈阳军区总医院 [3]吉林大学公共卫生学院 [4]苏州大学放射医学与公共卫生学院放射生物教研室 [5]吉林大学基础医学院生物化学教研室 [6]吉林大学中日联谊医院

出　　处：《中华放射医学与防护杂志》2008年第6期606-608,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30600160）

摘　　要：目的探讨Eg-IFNγ基因治疗联合放射性核素^125Ⅰ-脱氧尿嘧啶核苷治疗方案在荷H22肝癌细胞小鼠体内抑瘤效应及机制。方法小鼠肿瘤局部注射脂质体包裹的质粒，注射后48h，肿瘤局部注射370kBq ^125Ⅰ-UdR。观察各组小鼠治疗后不同时间肿瘤生长率；治疗后第3天，检测肿瘤胞浆蛋白中IFNγ的表达和脾脏CTL细胞毒活性。结果基因-放射核素治疗后第6～15天，pcDNAEg-IFNγ＋^125Ⅰ-UdR组肿瘤生长率明显低于对照组、^125Ⅰ-UdR组及pcDNAEgr-1＋^125Ⅰ-UdR组；基因-放射性核素治疗后第3天，pcDNAEgr-IFNγ＋^125Ⅰ-UdR组肿瘤胞浆蛋白中可检测到IFNγ的表达，其余组肿瘤胞浆蛋白中未检测到IFNγ的表达；pcDNAEgr-IFNγ＋^125Ⅰ-UdR组小鼠脾脏CTL细胞毒活性明显高于其余组（P〈0．01）。结论pcDNAEgr-IFNγ基因治疗联合放射性核素^125Ⅰ-UdR治疗抑瘤效应明显优于单纯^125Ⅰ-UdR放射性核素治疗。Objective To explore the anti-tumor effects of Egr-IFNγ gene therapy combined with ^125Ⅰ-UdR radionuclide therapy in mice bearing H22 hepatocarcinoma and its mechanism. Methods The recombinant plasmid pcDNAEgr-IFNγ mixed with liposome was injected into tumor. 48 h later, 370 kBq ^125Ⅰ-UdR was injected into tumor. The tumor growth rates at different times were observed. After 3 d generadionuclide therapy, the concentration of IFNγ in cytoplasm of H22 cells and cytotoxic activities of splenic CTL of the mice in different groups were examined. Results The tumor growth rates of pcDNAEgr-IFNγ ＋ ^125Ⅰ-UdR group were obviously lower than those of control group, ^125Ⅰ-UdR group and pcDNAEgr-1 ＋ ^125Ⅰ-UdR group 6-15 d after gene-radionuclide therapy. IFNγ protein was found in cytoplasm of H22 cells in pcDNAEgr-IFNγ ＋ ^125Ⅰ-UdR group after 3 d gene-radionuclide therapy. Cytotoxic activity of splenic CTL in pcDNAEgr-IFNγ ＋ ^125Ⅰ-UdR group was significantly higher than that in the other groups （ P 〈 0.01 ）. Conclusions The anti-tumor effects in vivo of pcDNAEgr-IFNγ gene therapy combined with ^125Ⅰ-UdR radionuclide therapy are better than those of ^125Ⅰ-UdR therapy.

关 键 词：Γ干扰素 H22细胞 基因-放射性核素治疗 脱氧尿苷 碘放射性同位素 

分 类 号：R285.5[医药卫生—中药学]