肠功能恢复汤对肠缺血-再灌注损伤大鼠肝肾肺功能的影响  

Effect of decoction to help recover intestinal function on hepatic,renal and pulmonary functions of rats with gut ischemia-reperfusion injury

作　　者：王春荣[1] 程永刚[1] 席孝忠[1] 田苑[1] 

机构地区：[1]西安交通大学医学院第一附属医院普通外科,陕西西安710061

出　　处：《第四军医大学学报》2008年第24期2240-2243,共4页Journal of the Fourth Military Medical University

基　　金：陕西省科学技术厅计划项目(2007K16-07)

摘　　要：目的:观察肠功能恢复汤对肠缺血-再灌注损伤大鼠肝、肾和肺功能的影响.方法:SD大鼠45只,随机分为正常对照组,肠缺血-再灌注损伤模型组(模型组),肠功能恢复汤治疗组(治疗组).分别检测各组血清中丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),总蛋白(TP),总胆红素(TB),肌酐(Cr)和尿素氮(BUN)含量;测定肺系数、肺组织湿干质量比值(W/D),髓过氧化物酶(MPO)活性和动脉血氧分压(PaO2);取肠系膜静脉血、肠系膜淋巴结、肝、肾及肺组织进行细菌培养,观察细菌移位情况,取肝、肾和肺组织行HE染色光镜观察.结果:治疗组与模型组相比较,血清中ALT,AST,TB,Cr和BUN含量以及肺系数、肺组织W/D,MPO活性、细菌移位率均降低(P<0.05),PaO2升高(P<0.05);TP三组间无差异(P>0.05).治疗组肝、肾和肺组织的病理学损伤程度明显小于模型组.结论:肠功能恢复汤对肠缺血-再灌注损伤大鼠的肝、肾和肺功能具有保护作用.AIM： To investigate the effect of the decoction to help recover intestinal function on hepatic, renal and pulmonary functions of rats with gut ischemia-reperfusion injury. METHODS： Forty-five SD rats were divided into 3 groups randomly： control group, gut ischemia-reperfusion injury group and decoction group. The contents of alanine transaminase （ALT）, aspartate transaminase （ AST ） , total protein （ TP ）, total bilirubin（ TB）, creatinine（ Cr ） and blood urea nitrogen （ BUN ） in serum, lung coefficient, the lung W/D ratio, the activity of myeloperoxidase（MPO） in lung tissue, PaO2 in each group were detected. We also observed the bacteria translocation and the pathomorphologieal changes of liver, kidney and lung. RESULTS： Compared with gut ischemia-reperfusion injury group, the contents of ALT, AST, TB, Cr and BUN in serum and bacteria transloeation ratio, lung coefficient, the lung W/D ratio, the activity of MPO in lung tissue were lower （ P 〈 0.05 ） , and PaO2 was higher（P 〈 0.05 ）in the decoction group. There was no significant difference in TP between 3 groups. The liver, kidney and lung of decoction group were better than those of gut ischemia-reperfusion injury group in pathomorphology. CONCLUSION： The decoction to help recover intestinal function can protect the hepatic, renal and pulmonary functions of rats with gut ischemia-reperfusion injury from damage.

关 键 词：肠功能恢复汤 肠缺血 再灌注损伤 肝功能试验 肾功能试验 呼吸功能试验 

分 类 号：R285.5[医药卫生—中药学]