樟树几种化学类型及近缘种的ISSR分析  

ISSR Analysis on the several chemical types of Cinnamomum Camphora and Sibling Species

作　　者：邢建宏[1] 陈存及[2] 张国防[2] 刘希华[1] 梁一池[1] 

机构地区：[1]三明学院,福建三明365004 [2]福建农林大学林学院,福建福州350002

出　　处：《福建林业科技》2008年第4期101-104,共4页Journal of Fujian Forestry Science and Technology

基　　金：福建省林业厅林木种苗攻关项目资助(200306)

摘　　要：利用ISSR分子标记分析了樟树3种化学类型及3个近缘种的亲缘关系和遗传多样性，从40条ISSR引物中筛选到具有多态性的引物15条用于PCR扩增，共扩增出133条DNA带，多态性条带比率为58．65％。供试样品的遗传相似系数变化范围在0．108～0．986之间。对35个样品进行聚类分析结果表明，所选材料在GD（遗传距离）约为0．21处可划分为樟树、油樟、云南樟、猴樟4大类群，在GD约为0．18处可将樟树的几种化学类型明显划分为芳樟、桉樟、脑樟3类，表明樟树种内具有丰富的遗传变异。The genetic diversity and phylogenetic relationship of three chemical types of Cinnamomum Camphora and its three sibling species were analyzed by ISSR. The results showed that a total of 133 loci were detected using 15 primers selected from 40 IS- SR primers, and the polymorphism rate was 58.65 % . Genetic similarity values between samples varied from 0. 108 to 0. 986. According to the results of clustering analysed with the genetic distance among 35 samples, the materials selected can be divided into 4 major groups, C. camphora, C. longepaniculaturn, C. glanduliferum and C. bodinieri, while the GD （genetic distance） is about 0.21. When the GD is about 0.18, the several chemical types of C. camphora can be divided into Linalool, Cineol and Camphor types. This shows that the genetic variance of C. camphora are rich.

关 键 词：樟树 分子标记 ISSR 

分 类 号：Q813.4[生物学—生物工程] S792.23[农业科学—林木遗传育种]