多棘海盘车的化学成分  

Study on chemical constituents of Starfish Asterias amurensis

作　　者：程萍[1] 汤华[2] 朴淑娟[1] 陆祎[1] 王增蕾[2] 林厚文[1] 

机构地区：[1]第二军医大学长征医院药学部,上海200003 [2]第二军医大学药学院海洋药物研究室,上海200433

出　　处：《第二军医大学学报》2008年第12期1479-1482,共4页Academic Journal of Second Military Medical University

基　　金：国家高新技术研究发展计划(“863”计划)(2006AA09Z423);长征医院“三重三优”学科人才建设计划~~

摘　　要：目的:研究多棘海盘车正丁醇萃取部位的化学成分。方法:利用溶媒萃取和多种色谱法,对多棘海盘车的正丁醇萃取部位化学成分进行分离,根据理化性质和现代波谱技术鉴定化合物的化学结构,并测试了其中5个化合物的生物活性。结果:分离并鉴定了5个化合物,分别为多羟基甾体皂苷amurensoside D(Ⅰ)、色氨酸(Ⅱ)和3个硫酸甾醇,包括3β-O-硫酸酯化-6α,23β-二羟基-胆甾-9(11)-23-羰基-硫酸盐(Ⅲ)、3β-O-硫酸酯化-6α-羟基-胆甾-9(11),20(22)-二双键-23-羰基-硫酸盐(Ⅳ)和3β-O-硫酸酯化-6α-羟基-麦角甾-9(11)-双键-23-羰基-硫酸盐(Ⅴ)。结论:化合物Ⅱ是首次从多棘海盘车中得到,化合物Ⅲ和Ⅴ对稻瘟霉菌P-2b显示较强的药理活性。Objective： To investigate the chemical constituents in the BuOH extract of Asterias arnurensis. Methods： The compounds were obtained by a combination of extraction and purification with suitable solvent and multiple column chromatographies; their structures were determined based on spectral analysis and physicochemical properties. The hioactivities of 5 compounds were tested. Results： Five compounds were isolated and identified, and they were amurensoside D （ Ⅰ ）, tryptophan Ⅱ , and 3 sulfated sterols, including 3β-O-sulfated-6α, 20β-diol-cholest-9 （ 11 ） en-23-one sodium salt （ Ⅲ ）, 3β-O- sulfated-6α-ol-cholesta-9 （ 11 ）, 20 （ 22 ）-dien-23-one sodium salt （ Ⅳ ）, and 3β-0-sulfated-6α-ol-ergost 9 （ 11 ）-en-23-one sodium salt （Ⅴ）. Conclusion： TryptophanⅡ is obtained from this species for the first time; compoud Ⅲ and compound V exhibit potent inhibitory activity against Pyricularia oryzae P 2b.

关 键 词：多棘海盘车 皂苷 硫酸甾醇 

分 类 号：R284.1[医药卫生—中药学]