催乳素调节鸡卵泡膜细胞功能的机理  

Effective Mechanism of Prolactin on the Follicular Theca Cells from Domestic Fowl

作　　者：李莹辉[1] 汪琳仙[1] 杨传任 

机构地区：[1]中国农业大学生物学院

出　　处：《中国兽医学报》1998年第1期77-80,共4页Chinese Journal of Veterinary Science

摘　　要：在明确催乳素（ＰＲＬ）对鸡卵泡膜细胞功能调节作用的基础上，探讨了酪氨酸激酶活性，ｃＡＭＰ系统和钙离子在ＰＲＬ调节膜细胞功能中的作用。结果表明，酪氨酸激酶抑制剂Ｇｅｎｉｅｓｔｉｎ能部分阻断ＰＲＬ对ＦＳＨ促膜细胞雌二醇（Ｅ２）合成的抑制作用，磷酸二酯酶抑制剂ＭＩＸ亦能部分消除ＰＲＬ对ＦＳＨ促Ｅ２合成的抑制作用，而细胞外钙离子浓度和电压依赖式钙通道在ＰＲＬ调节的膜细胞功能中无明显作用。The action of tyrosine kinase activity, cAMP system and calcium ion on the effect of PRL on steroidogenesis of theca cells were studied. The results indicated that geniestin, a tyrosine kinase inhibitor, partly abolished the inhibition of PRL on FSHinduced estradiol (E2) production suggesting that tyrosine activity is involved in the effect of PRL on FSHinduced E2 production. The inhibitory influence of PRL on FSHinduced steroidogenesis reduced when the 3isobutylmethy1xanthine (MIX), a inhibitor of phosphodiesterase (PDE), exists. This effect of PRL may also be act by enhancing PDE activity and inhibiting cAMP accumulation. The extracellular calciun ion concentration and voltageregulated calcium channels showed no significant effect on the steroidogenesis of theca cells regulated by PRL.

关 键 词：鸡 卵泡膜细胞 催乳素 酪氨酸激酶 磷酸二酯酶 

分 类 号：S852.2[农业科学—基础兽医学] S831.1[农业科学—兽医学]