Identification of differential gene expression for microarray data using recursive random forest  

作　　者：WU Xiao-yan WU Zhen-yu LI Kang 

机构地区：[1]Department of Biostatistics, College of Public Health, Harbin Medical University, Harbin, Heilongjiang 150081, China

出　　处：《Chinese Medical Journal》2008年第24期2492-2496,共5页中华医学杂志（英文版）

基　　金：The project was supported by a grant from the National Natural Science Foundation of China （No. 30371253）.Acknowledgement： We sincerely appreciate the comments from Edgar J. Love.

摘　　要：Background The major difficulty in the research of DNA microarray data is the large number of genes compared with the relatively small number of samples as well as the complex data structure. Random forest has received much attention recently; its primary characteristic is that it can form a classification model from the data with high dimensionality. However, optimal results can not be obtained for gene selection since it is still affected by undifferentiated genes. We proposed recursive random forest analysis and applied it to gene selection. Methods Recursive random forest, which is an improvement of random forest, obtains optimal differentiated genes after step by step dropping of genes which, according to a certain algorithm, have no effects on classification. The method has the advantage of random forest and provides a gene importance scale as well. The value of the area under the curve （AUC） of the receiver operating characteristic （ROC） curve, which synthesizes the information of sensitivity and specificity, is adopted as the key standard for evaluating the performance of this method. The focus of the paper is to validate the effectiveness of gene selection using recursive random forest through the analysis of five microarray datasets; colon, prostate, leukemia, breast and skin data. Results Five microarray datasets were analyzed and better classification results have been attained using only a few genes after gene selection. The biological information of the selected genes from breast and skin data was confirmed according to the National Center for Biotechnology Information （NCBI）. The results prove that the genes associated with diseases can be effectively retained by recursive random forest. Conclusions Recursive random forest can be effectively applied to microarray data analysis and gene selection. The retained genes in the optimal model provide important information for clinical diagnoses and research of the biological mechanism of diseases.Background The major difficulty in the research of DNA microarray data is the large number of genes compared with the relatively small number of samples as well as the complex data structure. Random forest has received much attention recently; its primary characteristic is that it can form a classification model from the data with high dimensionality. However, optimal results can not be obtained for gene selection since it is still affected by undifferentiated genes. We proposed recursive random forest analysis and applied it to gene selection. Methods Recursive random forest, which is an improvement of random forest, obtains optimal differentiated genes after step by step dropping of genes which, according to a certain algorithm, have no effects on classification. The method has the advantage of random forest and provides a gene importance scale as well. The value of the area under the curve （AUC） of the receiver operating characteristic （ROC） curve, which synthesizes the information of sensitivity and specificity, is adopted as the key standard for evaluating the performance of this method. The focus of the paper is to validate the effectiveness of gene selection using recursive random forest through the analysis of five microarray datasets; colon, prostate, leukemia, breast and skin data. Results Five microarray datasets were analyzed and better classification results have been attained using only a few genes after gene selection. The biological information of the selected genes from breast and skin data was confirmed according to the National Center for Biotechnology Information （NCBI）. The results prove that the genes associated with diseases can be effectively retained by recursive random forest. Conclusions Recursive random forest can be effectively applied to microarray data analysis and gene selection. The retained genes in the optimal model provide important information for clinical diagnoses and research of the biological mechanism of diseases.

关 键 词：MICROARRAY gene selection recursive random forest 

分 类 号：Q78[生物学—分子生物学]