肾间质损伤早期信号蛋白JAK_2/STAT_3与TGF-β_1表达趋势及相关性  

Trend of expression and relativity of JAK_2/STAT_3 signal protein and TGF-β_1 on early stage tubulointerstitial lesions

作　　者：郭虹[1] 马宏[2] 殷爱云[3] 

机构地区：[1]山西医科大学,山西太原030001 [2]山西医科大学第一医院,山西太原030001 [3]连云港市第二人民医院,江苏连云港222000

出　　处：《临床医药实践》2008年第12期963-965,共3页Proceeding of Clinical Medicine

摘　　要：目的:控讨幼年大鼠肾小管间质损伤早期,跨膜信号传导通路中JAK2/STAT3信号分子表达趋势及其与转化生长因子-1β(TGF-1β)表达的相关性。方法:以单侧输尿管梗阻(UUO模型)幼年大鼠为实验模型;采用免疫组织化学方法检测,行UUO术后3,7,14,28 d大鼠肾组织JAK2/STST3及TGF-1β蛋白的表达,并从时相上评价JAK2/STAT3与TGF-1β表达的相关性。结果:UUO组3 d各指标表达强度即增加,随着时间的延续,三者呈同步上调趋势。各时间点与对照组比较有统计学差异(P<0.01);UUO组内各时间点间比较有统计学意义(P<0.01),各指标两两之间的相关系数r值均大于0,且呈高度正相关关系。结论:UUO大鼠模型肾小管间质损伤早期,JAK2/STST3及TGF-1β间可能存在一条促肾小管质损伤的信号途径。Objective：To investigate the trend of expression and relatinvity of JAK2/STAT3 signal protein and TGF-β1 on early stage tubulointerstitial lesions in young rats of unilateral ureteral obstruction（UUO）. Methods ：Male young wistar rats with unilateral ureteral obstruction were used as experimental models. The JAK2/STAT3 and TGF-β1 protein expression in renal tissue were detected by immunohistochemical ways on days 3,7,14 and 28 after UUO and the correlation among JAK2/STAT3and TGF-β1 protein expression in time phase were evaluated. Resuhs：Immunohistochemical staining indicated that the level of expression of the three proteins were increased at day 3 in UUO group compared with control group. And they were up-regulated simultaneously in tubular epithelial cells. The significant difference were observed between control groups and UUO groups in different time points （P〈0.01）. Positive correlations were observed among the three factors （P〈0.01）. Conclusion：There might be a biologic signalpathway among JAK2/STAT3and TGF-β1 in the early phase of. tubulointerstitial lesions induced by unilateral ureteral obstruction in young rats ,also,it might be one of mechanism that enhanced tubulointerstitial nephropathy.

关 键 词：肾小管间质纤维化 JAK2/STST3 转化生长因子-Β1 

分 类 号：R692[医药卫生—泌尿科学]