磷酸二酯酶在鼠胸主动脉中的表达及其对血管张力的调节作用  

Expression of phosphodiesterase in rat thoracic aorta and its effects on vascular tone

作　　者：周海燕[1] 张冠楠[1] 李宁宁[1] 高平进[1] 

机构地区：[1]上海交通大学医学院瑞金医院上海市高血压研究所上海市血管生物学重点实验室,上海200025

出　　处：《上海交通大学学报（医学版）》2008年第12期1519-1522,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30540420525);上海交通大学医学院博士点建设基金(BXJ0740)~~

摘　　要：目的观察磷酸二酯酶（PDE）1和PDE3亚型在大鼠胸主动脉中的表达及其对血管张力的调节作用。方法采用RT-PCR检测PDE1和PDE3亚型mRNA的表达。将大鼠胸主动脉制备成2—3mm的血管环，苯肾上腺素（PE）诱导血管环收缩后，分别用特异性PDE1抑制剂IC86340和PDE3抑制剂milrinone处理去除内皮组和保留内皮组的血管环，测定两组舒张血管的作用。结果①PDE1A、PDE1B、PDE1C、PDE3A和PDE3B亚型mRNA在大鼠胸主动脉均有表达。②在保留内皮组和去除内皮组，IC86340均舒张PE诱发的血管收缩（P〈0．01）；并且对去除内皮组的舒张作用小于保留内皮组（P〈0．01）。③在保留内皮和去除内皮组，milrinone均可舒张PE诱发的血管收缩（P〈0．01）；但milrinone在低浓度时（1×10^-6.5～1×10^-6mol／L），去除内皮组舒张血管的作用大于保留内皮组（P〈0．05），而高浓度（1×10^-5.5 ～1×10^-5mol／L）时两组差异无统计学意义（P〉0．05）。结论PDE1和PDE3亚型mRNA在大鼠胸主动脉表达并参与血管张力的调节。PDE1部分通过血管内皮依赖的机制调节血管张力，PDE3则通过内皮非依赖的途径调节血管张力。Objective To observe the expression of phosphodiesterase （PDE） 1 and PDE3 in rat thoracic aorta and its effects on vascular tone. Methods The expression of PDE1 and PDE3 mRNA was detected by RT-PCR. Rat thoracic aorta was cut by 2 to 3 mm for vascular rings. Following the vascular constriction induced by phenylephrine （PE） , specific PDE1 inhibitor IC86340 and PDE3 inhibitor milrinone were administrated, and the relaxing forces were measured with or without endothelia. Results The expression of PDE1A, PDE1B, PDE1C, PDE3A and PDE3B mRNA was detected in rat thoracic aorta. IC86340 relaxed vascular ring tone with or without endothelia （P 〈 0.01 ） , and the relaxing effect on blood vessel with endothelia was greater than that on blood vessel without endothelia （P 〈0.01 ）. Milrinone relaxed vascular ring tone with or without endothelia （P 〈0.01 ）. The relaxing effect of milrinone at a lower dose（ 1 × 10^-6.5 ～ 1 × 10^-6 mol/L） was lesser on blood vessel with endothelia than that on blood vessel without endothelia （P 〈 0.05） , while there was no significant difference with a higher dose of milrinone（1 ×10^-5.5 ～ 1 × 10^-5 mol/L）（P 〉 0.05）. Conclusion PDE1 and PDE3 mRNA are expressed in rat thoracic aorta and involved in the modulation of vascular tone. PDE1 regulates vascular tone partially in an endothelia-dependent manner, while PDE3 in an endothelia-independent manner.

关 键 词：磷酸二酯酶 胸主动脉 血管内皮 血管张力 

分 类 号：R654.3[医药卫生—外科学]