亚硝化细菌的分离及初步鉴定  

Isolation and Preliminary Identification of Nitrite Bacteria

作　　者：廖雪义[1] 蓝荣 

机构地区：[1]襄樊学院化学与生物科学系,湖北襄樊441053 [2]广东省中山市杨氏南北鲜果有限公司,广东中山528451

出　　处：《生物技术》2008年第6期50-52,共3页Biotechnology

摘　　要：目的:为了使短程硝化—反硝化生物脱氮工艺能更有效地应用于含氮污废水的处理,需分离出高效的亚硝化细菌。方法:采用亚硝化细菌富集培养基选择培养和硅胶平板分离法,通过初筛、复筛,从广西大学农场菜园土中分离到一株亚硝化速率较高的菌株N4。结果:菌株N4革兰氏染色阴性,不产芽孢,细胞短杆状,呈单个排列,有一根亚极端鞭毛。结论:初步鉴定菌株N4为亚硝化单胞菌属(Nitrosomonas sp)。ObJective： In order to effectively treat sewage and wastewater containing nitrogen by short- cut nitrification- denitrification technology, efficient nitrite bacteria must be isolated. Method： One strain N4, with ability of oxidizing ammonia was isolated from vegetable garden soil of Guangxi University with silica gel plates and enrichment cultivation. Result： The strain N4 was Gram- negative, non sporulating, short - rod shaped bacterium that singly arranged, it had one sublime flagellum. Condusion： Primarily identified the strain N4 belongs to Nitrosomanas.

关 键 词：亚硝化细菌 分离 鉴定 

分 类 号：Q93-331[生物学—微生物学]