伊布利特对兔心房细胞快钠电流活性的影响  

Effects of Ibutilide on I_(Na) current in isolated atrial myocytes of rabbit

作　　者：齐书英[1] 何振山[1] 姚浩洁[1] 王洁[1] 李洁[1] 杨莉[1] 

机构地区：[1]白求恩国际和平医院心内科,河北石家庄050082

出　　处：《中国心脏起搏与心电生理杂志》2008年第6期547-550,共4页Chinese Journal of Cardiac Pacing and Electrophysiology

摘　　要：目的观察不同浓度伊布利特对心房细胞快钠通道的影响。方法新西兰纯种大耳白兔40只,随机分为正常对照组(Con),伊布利特10-7mol/L、10-6mol/L和10-5mol/L浓度组。利用胶原酶酶解法分离心房肌细胞,应用膜片钳全细胞记录方法,记录对照组及伊布利特3种浓度组心房细胞INa活性,并与正常对照组进行比较分析。结果INa峰值电流密度在对照组为-87.12±4.67pA/pF;伊布利特10-7mol/L、10-6mol/L和10-5mol/L组分别为-68.82±4.43,-45.26±3.46和-33.46±3.11pA/pF,伊布利特组较对照组有明显下降(P<0.05或0.01),伊布利特3组之间也有显著差异且呈浓度依赖性(P<0.05)。伊布利特组的INa稳态失活曲线浓度依赖性左移,INa再恢复明显减慢。结论伊布利特浓度依赖性的抑制心房细胞0相快钠电流,这可能是伊布利特治疗房性心律失常的部分离子通道机制。Objective To probe into the ion channel mechanism of Ibutilide antiarrythmie potential at eelluar level, by studying the sodium current （ INa ） of atrial cells of rabbit. Method New Zealand white rabbits were selected. Their hearts were cannulated via aorta and recorded on a Langendorff apparatus and perfnsed laterly. Single atrial cells were isolated enzymatically from rabbit left free wall, and INa was recorded by using patch clamp technique in the whole cell configuration before and after perfusiong of 10^-7、10^-6 and 10^-5mol/L lbutilide solutions（ 10 ^-7, 10 ^-6 and 10 ^-5 group）. Result The peak INa density of atrial cells in control group, 10 ^-7, 10 ^-6 and 10^-5 Ibutilide group were - 87.12 ± 4.67, - 68.82 ± 4.43, -45.26± 3.46 and -33.46 ± 3.11 pA/pF, respectively. The INa Ⅰ-Ⅴ curves of Ibutilide groups decreased significantly （ P 〈0.05 or 0.01 ）. The steady-state inactivation curves of the two Ibutilide groups shifted to the hyperpolarizing direction compared with the control group （ P 〈 0.05 ）. The INa recovery curve from inactivation recovered more slowly in Ibti- lide groups. Conclusion Ibutilide concentration-dependently inhibits IN, of normal atrial cells of rabbit, which maybe explains the mechanism of its antiarrhythmic effect.

关 键 词：电生理学 伊布利特 心房细胞 膜片钳 离子通道 快钠电流 

分 类 号：R331.38[医药卫生—人体生理学] R972.2[医药卫生—基础医学]