硫化镍对NF-KB基因表达的影响  

Influence of nickel purite on expression of NF-KB gene

作　　者：王婷[1] 朱玉真[1] 段舞云 毛英婷 王振萍 谢玉芳 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]金川有色金属公司职工医院

出　　处：《毒理学杂志》2008年第6期435-436,共2页Journal of Toxicology

基　　金：教育部博士点专项科研基金(20050730031)

摘　　要：目的探讨硫化镍对核转录因子KB(NF-KB)基因表达的影响。方法人支气管上皮细胞(16HBE)给予低、中、高剂量的硫化镍处理后,使用反转录-多聚酶链式反应(RT-PCR)法检测出人支气管上皮细胞(16HBE)中NF-KB的表达,分别在4、8、12和24 h后检测其NF-KB表达的动态变化。结果与生理盐水组比较,硫化镍(中、高剂量组)对NF-KB基因呈高表达,硫化镍(低剂量组)对NF-KB表达的影响与对照组比较,差异无统计学意义(P>0.05)。结论硫化镍(中,高剂量)能明显激活NF-KB的表达,可能是硫化镍致癌的分子机制之一。Objective To study influence of nickel purite on expression of NF-KB gene. Methods We added into low, middle, high doses Of nickel purite in human bronchus epithelial cell（ 16HBE）, we measured the expression of NF-KB gene by the way of RT-PCR. Results The expression of NF-KB gene in higher nickel purite groups increased significantly than that in NS group （ P 〈 0.01 ）. Lower nickel pttrite Group could not increased the expression of NF-KB（ P 〈 0.05）. Conclusion The excessive expression of NF-KB gene could be one of molecular mechanism of nickel purite carcinogenesis.

关 键 词：硫化镍 核转录因子KB 人支气管上皮细胞 

分 类 号：R99[医药卫生—毒理学]