雷公藤多甙对IL-1β诱导大鼠滑膜细胞株RSC-364表达JNK1蛋白的影响  

The Effect of Tripterygium Glycosides on the Expression of JNK1 in Rat Synovial Cell Line RSC-364 Stimulated with IL-1β

作　　者：周蓓[1] 蔡安季[1] 苏卓娃[1] 

机构地区：[1]广东医学院附属深圳南山医院中心实验室,深圳518052

出　　处：《热带医学杂志》2008年第12期1220-1221,1224,共3页Journal of Tropical Medicine

基　　金：深圳市科技和信息局项目(No.200405067)

摘　　要：目的探讨雷公藤多甙(Tripterygium Glycosides,TG)对IL-1β诱导大鼠滑膜细胞株RSC-364JNK1的影响,进一步阐明雷公藤多甙治疗类风湿性关节炎的作用机制。方法运用Western-blot检测不同浓度的雷公藤多甙(0、5、10、20mg/L)对IL-1β诱导大鼠滑膜细胞株RSC-364JNK1蛋白的表达。结果雷公藤多甙明显地抑制IL-1β诱导大鼠滑膜细胞株RSC-364对JNK1蛋白的表达。结论雷公藤多甙抑制IL-1β诱导大鼠滑膜细胞株RSC-364对JNK1蛋白的表达,可能是其治疗类风湿性关节炎的重要作用机制之一。Objective To investigate the effect of Tripterygium Glycosides （TG） on e-Jun N-terminal kinase （JNK1） expression of rat synovial cell line RSC-364 stimulated with interleukin-1 beta（IL-1β） and to illuminate the mechanism of TG in the treatment of rheumatoid arthritis （RA）. Method The expression of JNK1 of the rat synvoial cell line RSC-364 stimulated with IL-1β induced by different doses of TG（0, 5, 10, 20mg/L） were detected by Western-blot. Result The expression of JNK1 of the rat synvoial cell line RSC-364 stimulated with IL-1β induced by TG were obviously suppressed. Conclusion The expression of JNK in the rat synovial cell line RSC-364 stimulated with IL-1β was inhibited by TG, which might be the potential mechanism in the treatment of RA.

关 键 词：雷公藤多甙 大鼠滑膜细胞株 JNK1 

分 类 号：R593.22[医药卫生—内科学]