中国淡水三角涡虫种群间的亲缘关系分析  

Phylogenetic relationship analysis of freshwater planarian (Dugesia) populations in China

作　　者：高艳[1] 周毛天[1] 黄诗笺[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《华中师范大学学报（自然科学版）》2008年第4期615-619,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家基础科学人才培养基金资助项目(J0630648).

摘　　要：利用RAPD分子标记技术对中国8个产地淡水三角涡虫种群进行亲缘关系分析．筛选出13个合适的随机引物对8产地涡虫种群基因组进行PCR扩增，共产生137个扩增片段，平均每个引物扩增出10．5个片段，片段大小在3002000bp之间．不同涡虫种群间遗传距离变化较大，种群间最大遗传距离为0．4818，最小遗传距离为0．0876，平均遗传距离为0．3845；UPGMA法聚类分析表明：8产地涡虫种群可聚为3个聚类群，其中贵州遵义凤凰山和江苏太湖东山岛的涡虫种群间亲缘关系最近，安徽合肥龙泉山的涡虫种群与其他产地种群的亲缘关系最远．本研究表明上述8产地三角涡虫种群有明显的遗传分化，且种群间的亲缘关系与地理距离没有显著相关性．The freshwater planarian populations collected from 8 localities in China were analyzed by RAPD. 13 primers were selected for PAPD PCR and produced 137 well-amplified fragments. Each primer produced 10. 5 bands on average and the sizes of the bands were from 300bp to 2000bp. The genetic distances between populations varied from 0. 0876 to 0. 4818. The average genetic distance was 0. 3845. UPGMA cluster analysis showed that the eight freshwater planarian populations grouped into 3 branches. The population collected from Mt. Fenghuang, Zunyi City, Guizhou Province and from Dongshan Island, Tai Lake, Jiangsu Province were closest in relationship, while fresh water planarian population collected from Mt. Longquan, Hefei, Anhui Province was the remotest in relation to all other populations. The research shows that RAPD technique is suitable for genetic diversity analysis of planarians. The 8 populations had clear genetic differentiation and there wasn＇t a strong link between genetic distance and geological distance.

关 键 词：淡水三角涡虫 RAPD 亲缘关系 

分 类 号：Q958[生物学—动物学]