家蚕感染浓核病毒(镇江株)晚期的中肠和血液组织蛋白变化  

Proteomic Changes of Midgut and Hemolymph of Silkworm in Advanced Stage of Infection by Bombyx mori Densovirus(Zhenjiang Strain)

作　　者：裘智勇[1,2] 李木旺[1,2] 沈兴家[1,2] 郭锡杰[1,2] 

机构地区：[1]中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,江苏镇江212018 [2]江苏科技大学,江苏镇江212003

出　　处：《蚕业科学》2008年第4期767-771,共5页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划“973”项目(编号2005CB12-1004);国家科技支撑计划项目(编号2006BAD06B03)

摘　　要：为了进一步探明家蚕浓核病毒(镇江株)对家蚕的致病机制,通过蛋白质双向电泳技术对家蚕感染浓核病毒晚期的中肠和血液组织蛋白进行了分析。结果表明,感受性蚕品种在感染浓核病毒晚期,其中肠、血液组织蛋白量急剧下降,有近30%的蛋白点消失,其余蛋白点的含量也大幅下调达50%以上。由此说明由于浓核病毒侵染破坏了家蚕中肠上皮组织以及中肠的消化吸收功能,阻断了营养物质的输入,进而极大地减少了蚕体自身组织蛋白的合成。Proteins extracted from the midgut and hemolymph of silkworm were analysed by two-dimensional polyacrylamide gel electrophoresis （2-DE） so as to understand the pathogenetic mechanism of Bombyx mon densovirus （Zhenjiang Strain, BmDNV-ZJ） to the silkworm. The results showed that the amount of proteins extracted from midgut and hemolymph of the silkworm decreased rapidly in the advanced stage of infection by BmDNV-ZJ. About 30% of the spots disappeared and the rest spots were down-regulated by more than 50%. This implies that the protein synthesis is significantly reduced due to destruction of the digestion and absorption functions of the midgut caused by the virus infection.

关 键 词：家蚕 浓核病毒 中肠 血液 组织蛋白 

分 类 号：S884.5[农业科学—特种经济动物饲养] Q51[农业科学—畜牧兽医]