E838联合γ射线对小鼠肝癌H22抑瘤作用的实验研究  

Effects of E838 in Combination with γ-ray on H22 Hepatocellular Carcinoma Cells of Mice

作　　者：吴红英[1] 王月英[1] 李德冠[1] 路璐[1] 孟爱民[1] 王彦[1] 王汝勤[1] 张良安[1] 史元[1] 

机构地区：[1]中国医学科学院放射医学研究所,天津市分子核医学重点实验室,天津300192

出　　处：《中国辐射卫生》2008年第4期387-388,共2页Chinese Journal of Radiological Health

基　　金：天津市自然科学重点基金项目(043802411)

摘　　要：目的探讨E838药物对小鼠-肝癌H22的抑瘤效果及合用137Cs γ射线是否具有抑瘤增效作用。方法取小鼠肝癌H22肿瘤组织经研磨用生理盐水稀释成约5×107/ml个肿瘤细胞,接种于小鼠腋下,0.2ml/只。实验分对照组、单放组、E838药物组(5、7.5、10mg/kg)和药物合用照射组及环磷酰胺组。E838各组每日1次ip给药,连续7d;环磷酰胺隔日1次共4次,药物合用照射组于给药的第4天进行全身1Gy照射,每日一次,连续5d。结果E8385、7.5、10mg/kg剂量组对小鼠H22的抑瘤率分别为50.6%、62.73%和54.82%,与对照组比较差异有显著性(P<0.01),小鼠骨髓有核细胞的数量均高于对照组,药物合用137Cs γ射线能提高抑瘤效果,对肿瘤的杀伤作用高于单放组和单药治疗组,抑瘤率分别为52.98%、67.61%和59.15%,结论E838对肝癌H22肿瘤细胞具有明显的抑制作用,合用γ射线具有抑瘤增效作用。Objective n this study, we investigated the inhibiting effects of combination treatment E838 with γ - ray on mouse liver cancer H22. Methods 0.2ml H22 cell suspension （5 × 10^7cells/ml）were subcutaneously was injected into flanks of IRM - 2 mice. The inoculated mice were invited into 5 groups ： control; simple E838 groups with dose at 5, 7.5 and 10 mg/kg, daily, total 7 times; simple cytoxan groups with dose at 25 mg/kg （3 times） ; only irradiated with 1 Gy γ-ray and E838 combined with γ- ray group. Results The tumor inhibiting rate for E838 treated group were 50.6% , 62.73% and 54.82% respectively, there were significant difference compared with control group（ P 〈0.01 ）. The tumor inhibiting rate for the combination group were 52.98 % , 67.61% and 59.15 % respectively （ P 〈 0.05, compared with control group ） , but there were no significantly difference between E838 groups and combination groups. Conclusion These data suggest that E838 have significant inhibiting effects for inoculated tumor in mouse. The tumor inhibition rate of combination groups seems to be higher than that of E838 treatment groups, but not significantly.

关 键 词：E838 肝癌H22 Γ射线 抑瘤作用 

分 类 号：R817.1[医药卫生—影像医学与核医学]