高功率微波辐射对大鼠POMC衍生肽和Hsp表达的影响  

Effects of High Power Microwave Radiation on Expression of POMC Derived Peptides and Heat Shock Protein 70 in Rats

作　　者：吕士杰 姜艳霞[1] 芦晓晶[1] 王程[1] 潘文干[1] 任旷[1] 

机构地区：[1]吉林医药学院生物化学与分子生物学教研室,吉林132013

出　　处：《中国辐射卫生》2008年第4期399-400,共2页Chinese Journal of Radiological Health

基　　金：吉林省科技厅白求恩基金项目(200705407)

摘　　要：目的探讨前阿黑皮素原(POMC)衍生肽和Hsp在高功率微波(HPM)辐射热应激损伤中作用及意义。方法采用HPM照射54只雄性Wistar大鼠,照射后6、24和48h活杀大鼠取材,采用放免和Western blot法,研究HPM辐射对大鼠β-Ep、ACTH和Hsp70含量的影响。结果HPM照射后6h,垂体β-Ep含量显著降低(P<0.01),血浆β-Ep和ACTH含量显著升高(P<0.05);照射后24h,血浆β-Ep含量恢复到正常水平,48h后ACTH含量恢复正常。100mW/cm2照射后,6和24h组Hsp70表达显著升高(P<0.05);200mW/cm2照射后24h,Hsp70表达显著升高(P<0.05)。结论HPM辐射可影响大鼠下丘脑-垂体神经内分泌网络功能和Hsp70表达,POMC衍生肽和Hsp参与了大鼠热应激损伤与修复的病理生理过程。Objective To research the fuction and significance of POMC derived peptides and Hsp 70 by stress damage of high power microwave radiation in rats. Methods 54 male Wistar rats were sacrificed at 6h ,24h and 48h after exposure to high power microwave radiation, using radioimmunity and westernblot to detect 15 - EP, ACTH and Hsp 70. Results The contents of β - EP in hypophysis decreased significantly （ P 〈 0.01 ）, β - EP （ P 〈 0.05 ） and ACTH （ P 〈 0.05 ） in serum increased markedly after 6h radiation; β - EP and ACTH in serum respectively returned to normal level after 24h and 48h . Hsp 70 expression increased significantly 6h and 24h after 100mW/cm^2 radiation （P 〈 0.05 ） ;24h after 200 mW/cm^2 radiation , Hsp 70 expression increased obviously（P 〈 0.05 ）. Conclusion High power microwave radiation can influence thalamencephalon -pituitary nerve - endocrine network function and Hsp70 expression, POMC derived peptides and Hsp participated in the pathophysiologic process of heat stress damage and repair

关 键 词：microwave Β-EP ACTH HSP 

分 类 号：R135.99[医药卫生—劳动卫生]