双柱吸附法对莲子心总碱的分离纯化研究  

ISOLATION AND PURIFICATION OF ALKALOIDS FROM PLUMULA NELUMBINIS BY DOUBLE-COLUMN ADSORPTION CHROMATOGRAPHY

作　　者：张吉祥[1] 郭金生[1] 杨佳玲[3] 欧来良[2] 

机构地区：[1]廊坊师范学院化学系,廊坊065000 [2]南开大学生物活性材料教育部重点实脸室,分子生物研究所,天津300071 [3]天津市产品质量监督检测技术研究院,天津300384

出　　处：《离子交换与吸附》2008年第6期512-517,共6页Ion Exchange and Adsorption

基　　金：河北省教育厅基金资助项目(No.2007317)

摘　　要：研究了大孔吸附树脂和阳离子交换树脂双柱吸附法对莲心碱、异莲心碱和甲基莲心碱的吸附行为。利用阳离子交换树脂D72制备出莲心碱、异莲心碱和甲基莲心碱,含量分别为10.6%、10.6%和28.0%,总含量为49.2%的莲子心总碱提取物,其中杂质主要是水溶性较大的生物碱成分。利用AKS-W大孔吸附树脂能去除极性较大的生物碱,而D72阳离子交换树脂能去除非生物碱的特点,采用双柱联合吸附法,制备出3种吸附质含量分别为33.1%、15.0%和34.5%,总含量为82.6%的莲子心总碱提取物。The performance of adsorption and separation for liensinine, isoliensinine and neferine was studied by double-column adsorption chromatography using maeroporous adsorption and cation exchange resins. The alkaloid extract with 49.2% total contents by mass representing 10.6% liensinine, 10.6% isoliensinine and 28.0% neferine respectively was prepared by D72 cation exchange resins, in which most of the impurities were water-soluble alkaloids. Furthermore, the alkaloid extract with 82.6% total contents by mass containing 33.1%, 15.0% and 34.5% of the three adsorbates respectively was prepared by double-column adsorption chromatography using AKS-W macroporous adsorption and D72 cation exchange resins. As a result, the content of single and total alkaloids has been greatly increased by the double-column adsorption chromatography.

关 键 词：双柱吸附法 大孔吸附树脂 阳离子交换树脂 莲子心总碱 

分 类 号：TQ425[化学工程]