青蒿提取物对伯氏疟原虫超微结构的影响  

EFFECTS OF QINGHAO EXTRACT ON THE ULTRASTRUCTURE OF PLASMODIUM BERGHEI

作　　者：田光[1] 李豫川[1] 王京燕[1] 纪晓光[1] 杨岚[2] 屠呦呦[2] 

机构地区：[1]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071 [2]中国中医科学院中药研究所,北京100700

出　　处：《寄生虫与医学昆虫学报》2008年第4期202-204,共3页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金项目(No.30572319)

摘　　要：采用4天抑制试验法,观察青蒿提取物和青蒿素对伯氏疟原虫超微结构的影响。结果显示,青蒿提取物和青蒿素均使原虫滋养体膜结构损伤,表现为虫体表膜、食物泡膜、限制膜、线粒体膜、内质网、核膜呈现肿胀及膜间隙增宽。有些虫体表膜、食物泡膜呈现多层螺纹膜样改变。严重病变的原虫滋养体退变崩解,结构消失,残留退变的食物泡和吞噬泡散在红细胞内。此外,还观察到青蒿提取物和青蒿素对疟原虫纳虫泡内裂殖子发育有抑制作用。试验证明,青蒿提取物和青蒿素对伯氏疟原虫滋养体的超微结构损伤部位和病变特征基本相同;并提示对疟原虫纳虫泡内裂殖子发育有一定影响。To observe the effect of Qinghao extract and artemisinine on the ultrastructure of Plasmodium berghei by Peters four-day oral treatment. The result showed membrane structure of the protozoon trophozoit was damaged by Qinghao extract and artemisinine. The polypide surface membrane, food vacuole membrane, restriction membrane, mitochondrial membrane, endoplasmic reticulum and nuclear membrane swelled. The intermembrane space widened. Some polypide surface membrane and food vacuole membrane showed multilayer thread-like change. Serve pathological changed protozoon trophozoit were cataplasis and their structure disappeared. The red blood cell contained the residual food vacuole and phagocytotic vesicle. The Qinghao extract and artemisinine had the inhibitory action on the development of merozoite in the plasmodium. Qinghao extract had the same effect on the damaged location of ultrastructure and character of pathological changes as the artemisinine. Meanwhile Qinghao extract and artemisinine had some effect on the development of the merozoite in the parasitophorous vacuole of plasmodium.

关 键 词：青蒿提取物 青蒿素 伯氏疟原虫 纳虫泡 超微结构 

分 类 号：R285.5[医药卫生—中药学]