真空、低能离子注入对人宫颈癌(HeLa)细胞中p53及c-fos mRNA的表达影响  

Effect of Vacuum and Low-energy Ion Implantation on p53 and c-fos mRNA Expression in HeLa Cells

作　　者：张凤秋[1] 方华圣[1] 押辉远[1] 秦广雍[1] 

机构地区：[1]郑州大学物理工程学院,河南郑州450052

出　　处：《原子核物理评论》2008年第4期385-390,共6页Nuclear Physics Review

基　　金：国家十五科技攻关资助项目(2001BA302B-03)~~

摘　　要：在具有高效护水功能石蜡油的保护下进行了人宫颈癌细胞(HeLa细胞)的低能离子注入及真空处理,用荧光定量PCR的方法分别研究了真空和30keVN+的不同注入剂量下,细胞中p53基因和c-fos基因的mRNA表达变化。结果表明,上述两个基因的表达与真空及低能离子注入之间存在着剂量关系。The relationship between gene expression and low-energy ion implantation has been studied.Because vacuum is prerequisite in low-energy ion implantation, mineral oil was used to protect cells from water evaporating. In this study, HeLa cells were implanted by low-energy ions （30 keV N^＋） at different doses, and p53 gene and c-fos gene were studied with real-time quantitative PCR. The result shows that gene expression changed obviously when cells were acted as vacuum control to the sample implanted 5×10^14ions/cm^2.

关 键 词：真空 低能离子注入 HELA细胞 基因表达 实时定量PCR 

分 类 号：Q274[生物学—细胞生物学] Q291