低能离子注入E.coli K12的HRS／IRR效应及recA基因在其诱发中的作用  

HRS/IRR in E. coli K12 Implanted by Low Energy Ion Beam and Effect of recA Gene on HRS/IRR

作　　者：杨天佑[1,2] 李培睿[2] 田静[1] 李宗伟[2] 秦广雍[2] 

机构地区：[1]河南科技学院生命科技学院,河南新乡453003 [2]郑州大学离子束生物工程省重点实验室,河南郑州450052

出　　处：《原子核物理评论》2008年第4期397-401,共5页Nuclear Physics Review

基　　金：国家重点基础研究发展规划资助项目(2004CB719604);国家自然科学基金资助项目(10505018)~~

摘　　要：以MG1655(野生型),LE392(recA-)和DH5α(recA-)3株E.coliK12菌株为材料,研究了30keVN+离子注入E.coliK12时HRS/IRR效应的诱发情况及recA基因在其诱发中的作用。结果显示:小于10×1014ions/cm2低剂量离子注入大肠杆菌可诱发HRS/IRR效应;30keVN+离子注入MG1655,LE392菌株都可诱发HRS/IRR效应,而在DH5α菌株中无法诱导IRR效应。recA-与HRS/IRR效应相斥性表明recA基因在HRS/IRR效应的诱发中发挥了重要作用。The HRS/IRR in Escherichia coli had been investigated with E. coli K12 wild strain MG1655 irradiated by the 30 keV N^＋. The curve of the dose-survival effect showed the 30 keV N^＋ ion could induce the HRS/IRR at the dose less than 10×10^14ions/cm^2. Moreover, the effect of recA gene in HRS/IRR inducement had been researched with three E.coli K12 strains, wild strain MG1655, LE392 （recA^＋） and DH5α（recA^-）. The results showed the IRR was disappeared in recA^- DH5α strain, but the HRS/IRR appeared in wild strain MG1655 and recA^＋ LE392, which suggested that recA gene and the DNA damages their repairs including recA gene were essential element in HRS/IRR inducement.

关 键 词：HRS/IRR N^+ 注入 E.COLI K12 RECA 

分 类 号：O53[理学—等离子体物理]