Preparation and preliminary evaluation of [^(55)Co](II)vancomycin  

作　　者：Amir Reza JALILIAN Yousef YARI-KAMRANI Pejman ROWSHANFARZAD Mahseed SABET Mohsen KAMALI-DEHGHAN Abbas MAJDABADI 

机构地区：[1]Nuclear Medicine Research Group, Agriculture, Medicine and Industrial Research School (AMIRS),Karaj,Iran

出　　处：《Nuclear Science and Techniques》2008年第6期347-353,共7页核技术（英文）

基　　金：Supported partly by IAEA/IAEO research grant for cyclotron production of Co-57.

摘　　要：Co-55 (t1/2=17.53 h) was produced by 150 μA irradiation of a natural nickel target using 15 MeV protons. It was separated from the irradiated target material by two ion exchange chromatography steps with a radiochemical yield of >95% and was used for the preparation of [55Co]vancomycin ([55Co]VAN). Optimization studies were per- formed using Co-57 due to its longer half-life. Cobalt-57 (t1/2=271.79 d) was produced by irradiation of a natural nickel target with 150 μA current of 22 MeV protons. The 57Co was separated from the irradiated target material using a no-carrier-added method with a radiochemical yield of >97%. Both products were controlled for radionuclide and chemical purity. The solutions of [55Co]VAN were prepared (radiochemical yield>80%) starting with 55Co acetate and vancomycin at room temperature after 30 min. A precise solid phrase extraction (SPE) method was developed using Si Sep-Pak in order to purify/reconstitute the final formulation for animal studies. [55Co]VAN showed a radiochemical purity of more than 99%. The resultant specific activity was about 1.15 TBq/mmol. It is proved that the tracer is stable in the final product and in presence of human serum at 37°C up to 24 h. Biodistribution study of [55Co]VAN in normal rats was undertaken for up to 72 h.Co-55 （t1/2=17.53 h） was produced by 150 uA irradiation of a natural nickel target using 15 MeV protons. It was separated from the irradiated target material by two ion exchange chromatography steps with a radiochemical yield of 〉95% and was used for the preparation of [^55Co]vancomycin （[^55Co]VAN）. Optimization studies were performed using Co-57 due to its longer half-life. Cobalt-57 （t1/2=271.79 d） was produced by irradiation of a natural nickel target with 150 pA current of 22 MeV protons. The 57Co was separated from the irradiated target material using a no-carrier-added method with a radiochemical yield of 〉97%. Both products were controlled for radionuclide and chemical purity. The solutions of [^55Co]VAN were prepared （radiochemical yield〉80%） starting with 55Co acetate and vancomycin at room temperature after 30 min. A precise solid phrase extraction （SPE） method was developed using Si Sep-Pak in order to purify/reconstitute the final formulation for animal studies. [^55Co]VAN showed a radiochemical purity of more than 99%. The resultant specific activity was about 1.15 TBq/mmol. It is proved that the tracer is stable in the final product and in presence of human serum at 37℃ up to 24 h. Biodistribution study of [55Co]VAN in normal rats was undertaken for up to 72 h.

关 键 词：钴-55 万古霉素 医用回旋加速器 放射性同位素示踪 生物剂量学 

分 类 号：O627.8[理学—有机化学]