大黄对大鼠肾脏水通道蛋白2、4表达的影响  

Effect of Rhubarb on Expressions of Aquaporin-2 and-4 in Rat's Kidney

作　　者：鲍军强[1] 李锋[1] 张文生[1] 梁亮[2] 王文[1] 刘晓渭[1] 赵燕玲[1] 

机构地区：[1]第四军医大学西京医院,西安710032 [2]第四军医大学医学遗传学与发育生物学教研室

出　　处：《中国中西医结合杂志》2008年第12期1108-1111,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No．30572385)

摘　　要：目的探讨大黄对大鼠肾脏水通道蛋白2、4(AQP2、AQP4)表达的影响。方法32只SD大鼠随机分为正常对照组,大黄低、中、高剂量组,分别给予生理盐水不同剂量的大黄提取物(大黄总蒽醌)灌胃,测定大鼠6 h及24 h尿量,运用免疫组化、Western blot和RT-PCR法检测肾脏AQP2、AQP4蛋白及mRNA表达变化。结果与正常对照组比较,大黄高、中剂量组大鼠尿量明显增加,肾脏AQP2、AQP4蛋白表达及mR- NA表达明显下降,差异有统计学意义(均P<0.01);与正常对照组比较,大黄低剂量组大鼠尿量及肾脏AQP2、AQP4蛋白及mRNA表达变化不明显,差异无统计学意义。结论大黄能抑制大鼠肾脏AQP2、AQP4蛋白及mRNA表达水平,这可能是其利尿功效的机制之一。Objective To investigate the effect of rhubarb on expressions of aquaporin-2 and -4 （AQP2 and AQP4） in rat＇s kidney. Methods Thirty-two SD rats were randomly divided into 4 groups, the normal control group, and the three rhubarb groups medicated via gastrogavage with low, mid and high dose of rhubarb extract （total anthraquinone） respectively. The 6 h and 24 h urine volume were measured, and the protein and mRNA expressions of AQP2 and AQP4 in renal tissue were determined with immunohistochemistry, Western blot and RT- PCR. Results No significant difference between the control group and the low dose rhubarb treated group was found in urine volume, as well as in AQP2 and AQP4 protein and mRNA expressions. But the urine volume was obviously higher, the protein and mRNA expressions of AQP2 and AQP4 were markedly lower in rats after mid/high dose rhubarb medication respectively when compared with those in the normal controls （all P 〈 0. 01 ）. Conclusion Rhubarb can inhibit the protein and mRNA expressions of AQP2 and AQP4 in rats＇ kidney, which probably is one of the mechanisms of rhubarb for diuresis.

关 键 词：大黄 肾脏 利尿 水通道蛋白2 水通道蛋白4 

分 类 号：R285.5[医药卫生—中药学]