RGD多肽接枝聚(乳酸-羟基乙酸-L-赖氨酸)的制备与表征  

Preparation and characterization of RGD peptide modification of poly{(lactic acid)-co-[(glycolic acid)-alt-(L-lysine)]}

作　　者：严琼姣[1] 李世普[1] 殷义霞[1] 李娟[1] 

机构地区：[1]武汉理工大学生物材料与工程研究中心,湖北武汉430070

出　　处：《中南大学学报（自然科学版）》2008年第6期1190-1195,共6页Journal of Central South University:Science and Technology

基　　金：国家重点基础研究发展计划资助项目(2005CB623905)

摘　　要：以3S-[4-(苄氧羰基氨基)丁基]-吗啉-2,5-二酮和丙交酯为起始原料,制备一种新型的RGD多肽接枝聚(乳酸-羟基乙酸-L-赖氨酸)共聚物(PRGD)。采用核磁共振氢谱、氨基酸分析、接触角测试、MTT实验和环境扫描电镜对其结构和性能进行表征。研究结果表明:RGD接枝量为6.9～14.3μmol/g;PRGD膜和聚乳酸(PLA)膜的水接触角分别为43.63°和62.45°;PRGD膜表面黏附的嗅鞘细胞较PLA组活性高,细胞密度大,生长状态好。PRGD较PLA具有更好的亲水性和神经细胞亲和性,有望成为一种理想的神经修复材料。A novel RGD peptide modification of poly[LA-co-（GA-alt-Lys）] copolymer（PRGD） was prepared by lactide and 3S-[4-（benzyloxycarbonylamino）butyl]morpholine-2,5-dione（BMD） as starting materials. The structure and properties of poly[LA-co-（GA-alt-Lys）]/RGD were characterized by ^1H-NMR, AAA, contact angel measurement, MTT experiments and ESEM. The results show that GRGDY is coupled to poly[LA-co-（GA-alt-Lys）] at a concentration of 6.9-14.3 μmol/g. The water contact angels of PRGD films and PLA films are 43.63° and 62.45°, respectively. Olfactory ensheathing celts（OECs） on the PRGD films exhibit higher viability and the amount of OECs on the PRGD films are much larger than those on PLA films, and moreover, the cells stretch very well. The PRGD has better hydrophilicity and neural cell affinity than PLA, so it is promising in nerve tissue engineering application.

关 键 词：RGD 聚(乳酸-羟基乙酸-L-赖氨酸) 细胞亲和性 

分 类 号：TQ316.343[化学工程—高聚物工业]