ERK5调节成骨细胞功能的研究  

Study of ERK5 regulating osteoblast function

作　　者：程群[1] 朱汉民[1] 甘洁民[1] 缪应新[1] 施泓[1] 

机构地区：[1]复旦大学附属华东医院老年医学研究所,上海200040

出　　处：《中国骨质疏松杂志》2008年第12期845-848,共4页Chinese Journal of Osteoporosis

基　　金：上海市科委计划项目(04ZR14044)

摘　　要：目的研究ERK5在成骨细胞的表达及IL-6对其表达的调控,初步探讨ERK5在成骨细胞增殖分化中的作用。方法应用免疫印迹法检测人、大鼠、小鼠成骨细胞和MG-63细胞ERK5的表达;用IL-6作用于MG-63细胞,检测不同时间和剂量干预后ERK5和P-ERK5表达情况,构建ERK5 siRNA和空白对照质粒,转染MG-63细胞,IL-6干预细胞后检测各组细胞增殖率、碱性磷酸酶活性和骨钙素表达的差异。结果在人、大鼠、小鼠成骨细胞和MG-63细胞中均有ERK5的表达;IL-6可呈时间、剂量依赖性的提高ERK5的磷酸化;与对照组相比,ERK5 siRNA的成骨细胞IL-6刺激后增殖降低,骨钙素表达量下降且差异有统计学意义,而碱性磷酸酶活性的变化差异无显著性。结论成骨细胞ERK5信号通路的活性受IL-6调控,ERK5信号通路参与了成骨细胞的增殖分化。Objective To study the expression of ERK5 on human, rat, mouse osteoblasts and the regulation of IL-6 on ERK5 expression, preliminarily investigate the effect of ERK5 on proliferation and differentiation of osteoblast. Methods The protein expression of ERK5 on osteoblasts was confirmed by western blot. ERK5 and P- ERK5 were detected when different dose of IL-6 treated osteoblast during different time. ERK5 siRNA plasmids were constructed and transfected into MG-63 cells. MTI＇, ALP activity and osteocalcin expression were detected after treated with 10 nmol/L IL-6. Results ERK5 was expressed in human, rat, mouse osteoblasts. The phosphorylation level of ERK5 were increased by IL-6 on time and dose dependent manner. As compared to control ceils, ERK5 siRNA cells＇ MTF reduced, osteocalcin expression reduced （ P 〈 0.05） yet the ALP activity was no significant difference. Conclusion ERK5 signal pathway is regulated by IL-6 in osteoblast, and ERK5 signal pathway participate in the proliferation and differentiation of osteoblast.

关 键 词：成骨细胞 ERK5 IL-6 RNA干扰 增殖 分化 

分 类 号：R336[医药卫生—人体生理学]