通心络对脑缺血再灌注损伤后神经巢蛋白及VEGF表达的影响  

Effect of Tongxinluo on nestin and vascular endothehal growth factor mRNA expression in rat brain tissue after cerebral ischemia-reperfusion injury

作　　者：王立新 尹瑞雪[2] 孙景波 

机构地区：[1]广省中医院脑血管病中心,广东广州510120 [2]南方医科大学南方医院神经内科,广东广州510515

出　　处：《南方医科大学学报》2008年第12期2131-2135,共5页Journal of Southern Medical University

基　　金：国家自然科学基金青年科学基金(30400612)

摘　　要：目的探讨神经巢蛋白(nestin)和血管内皮细胞因子(VEGF)在脑缺血再灌注损伤后脑组织内的表达变化关系及中药通心络的影响。方法采用大鼠缺血再灌注损伤(MCAO)模型,分别应用荧光免疫组织化学方法、RT-PCR法观察缺血后3、7、14、21d缺血侧室管膜及室管膜下区(SVZ)、海马齿状回(HDG)神经巢蛋白、缺血病灶周围脑组织内VEGFmRNA的表达变化及二者关系。给予模型大鼠大、小剂量通心络灌胃,观察其对不同时间段上述指标表达变化的影响。结果神经巢蛋白阳性细胞荧光强度值、VEGFmRNA含量随缺血再灌注时间的延长,表达均增加,第7、l4、21天组与假手术组比较,差异具有显著性(P<0.05)。经通心络大小剂量治疗后,各组nestin阳性细胞荧光强度值、VEGFmRNA含量均高于脑缺血再灌注模型组,差异显著(P<0.05),而VEGFmRNA含量除第3天无差别(P>0.05)外,第7、14、21于均高于脑缺血再灌注模型组,差异显著(P<0.05),而以大剂量组效果更为显著。结论大鼠缺血再灌注损伤后可引起其缺血侧SVZ、HDG区神经干细胞反应和增殖,其机理可能与其促进缺血灶周围的VEGFmRNA表达增加有关。Objective To investigate the expressions of nestin and vascular endothehal growth factor （VEGF） mRNAs in rat brain tissue after cerebral ischemia-reperfusion injury and their changes in response to Tongxinluo （a traditional Chinese herbal preparation） treatment. Methods Cerebral ischemia was induced in rats by temporary middle cerebral artery occlusion （MCAO） followed by treatment with Tongxinluo at high and low doses. On days 3, 7, 14 and 21 after MCAO, nestin and VEGF mRNA expressions in the ependyma, subventricular zone （SVZ）, and hippocampal subdentate gyrus zone （HDG） in the ischemic hemisphere were quantitatively analyzed using immunohistochemistry and RT-PCR. Results Compared with the sham-operated group, the rats with cerebral ischemia-reperfusion injury showed significantly increased nestin-positive neurons and VEGF mRNA expression in the SVZ and HDG 7, 14 and 21 days after MCAO （P〈0.05）. Treatment with Tongxinluo, especially at high doses, significantly increased the number of nestin-positive neurons and VEGF mRNA expression in the rats 7, 14 and 21 days after MCAO （P〈0.05）. Conclusion Focal cerebral ischemia in rats results in rapid response and proliferation of neural stem cells in the SVZ and HDG in the ischemic hemisphere possibly by increasing VEGF mRNA expression in the adjacent tissues around the ischemic focus. Tongxinluo may enhance the differentiation and proliferation capacity of the neural stem cells after MCAO by inducing the expression of VEGF mRNA.

关 键 词：脑缺血 神经巢蛋白 通心络 血管内皮细胞因子 

分 类 号：R285.5[医药卫生—中药学]