甘氨酸对新生大鼠离体延髓脑片呼吸节律性放电活动的调制  

Glycine is involved in the modulation of respiratory rhythmical discharge activity in neonatal rat medullary brain slices

作　　者：程静[1] 千智斌[1,2] 吴中海[1] 

机构地区：[1]南方医科大学生理学教研室,广东广州510515 [2]新乡医学院基础医学院,河南新乡453003

出　　处：《南方医科大学学报》2008年第12期2142-2145,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30570670);广东省自然科学基金(5004714)

摘　　要：目的探讨甘氨酸(Gly)在延髓基本节律性呼吸放电发生和调节中的作用。方法以改良Kreb's液恒温灌流新生Sprague-Dawley大鼠离体延髓脑片标本,稳定记录与之相连的舌下神经根的呼吸节律性放电活动(RRDA)后,第1、2组在灌流液中分别单独给予Gly受体的特异性激动剂Gly和特异性拮抗剂士的宁(STR);第3组分别先后给予Gly和Gly+STR,观察舌下神经根RRDA的变化,探讨Gly对其调节作用。结果给予Gly后,吸气时程和放电的积分幅度显著缩短,呼气时程和呼吸周期无明显变化;给予STR后,呼气时程和呼吸周期缩短,吸气时程和放电的积分幅度无明显变化,且Gly的作用可部分被STR逆转。结论Gly参与了哺乳动物基本呼吸节律的调节。Objective To determine the role of glycine （Gly） in the generation and modulation of basic respiratory rhythm. Methods Neonatal （0-3 days） SD rats of either sex were used in this study. The medulla oblongata brain slice containing the medial region of the nucleus retrofacialis （mNRF） and the hypoglossal nerve rootlets was prepared, and the surgical procedure was performed in the modified Kreb＇s solution （MKS） with continuous carbogen （95% O2 and 5% CO2） within 3 min. The rhythmical respiratory discharge activity （RRDA） of the hypoglossal nerve rootlets was recorded using suction electrode. Eighteen medulla oblongata slice preparations were divided into 3 groups and treated for 20 min with Gly receptor specific agonist Gly （10 μmol/L）, Gly receptor antagonist strychnine （STR, 1μmol/L）, or Gly＋STR after a 20 min Gly application. The changes in RRDA of the hypoglossal nerve rootlets were observed. Results Gly significantly decreased the inspiratory time and integral amplitude （IA）, but the changes of respiratory cycle （RC） and expiratory time （TE） were not statistically significant. STR induced a decrease in expiratory time and respiratory cycle without significantly affecting the inspiratory time or integral amplitud. The effect of Gly on the respiratory rhythm was partially reversed by additional application of STR. Conclusion Gly may play an important role in the modulation of RRDA in the medulla oblongata slice of neonatal rats.

关 键 词：甘氨酸 延髓 呼吸节律 节律性放电 

分 类 号：R338.2[医药卫生—人体生理学]