HLA-G在异基因造血干细胞移植中诱导免疫耐受  

Role of membrane-bound human leukocyte antigen G in inducing immune tolerance after allogeneic hematopoietic stem cell transplantation

作　　者：杜璇[1] 吴秀丽[1] 李蕊[1] 张钰[1] 范志平[1] 易正山[1] 刘启发[1] 

机构地区：[1]南方医科大学南方医院血液科,广东广州510515

出　　处：《南方医科大学学报》2008年第12期2202-2203,共2页Journal of Southern Medical University

基　　金：国家"863"高技术研究发展计划(2006AA02Z4A0);广东省自然科学基金(7005160)

摘　　要：目的研究供者粒细胞集落刺激因子(G-CSF)动员前后外周血和骨髓中单个核细胞人白细胞分化抗原G(HLA-G)膜表达以及血浆和骨髓液中HLA-G水平,揭示G-CSF动员骨髓移植优于外周血干细胞移植与骨髓中HLA-G有关的机制。方法流式细胞术检测外周血和骨髓中单个核细胞HLA-G膜表达以及酶联免疫吸附法(ELISA)检测血浆和骨髓液中HLA-G水平。结果动员后膜结合型HLA-G(mHLA-G)在外周血和骨髓中均显著高于动员前(P=0.001,P=0.000),且动员后骨髓mHLA-G含量较外周血中高(P=0.001)。动员后外周血中分泌型HLA-G(sHLA-G)较动员前无明显差异(P=0.279),动员后骨髓中sHLA-G较动员前明显增高(P=0.002),且动员后骨髓sHLA-G较动员后外周血中含量明显增高(P=0.004)。结论HLA-G在G-CSF动员后造血干细胞移植(HSCT)诱导免疫耐受中具有重要作用。Objective To evaluate the role of human leukocyte antigen G （HLA-G） in the better effect of allogenetic bone marrow transplantation than that of peripheral blood stem cell transplantation. Methods Flow cytometry was used to detect the expression of membrane-bound HLA-G （mHLA-G） on donor peripheral blood （PBC） or bone marrow （BM） mononuclear cells. The levels of soluble HLA-G （sHLA-G） in the plasma and bone marrow fluid were determined using enzyme-linked immunosorbent assay （ELISA） before and after granuloeyte colony-stimulating factor （G-CSF） mobilization. Results The mean levels of mHLA-G after G-CSF mobilization in the PBC and BM were significantly higher than that before G-CSF mobilization （P=0.001 and 0.000）, but the plasma levels of sHLA-G showed no significant changes after the mobilization （P=0.279）. The mean levels of sHLA-G in the BM fluid significantly increased （P=-0.002） to a level higher than that in the PBC after G-CSF mobilization （P=0.004）. Conclusion HLA-G plays an important role in immune tolerance after hematopoietic stem cell transplantation with G-CSF mobilization.

关 键 词：白细胞分化抗原 ALLO-HSCT 免疫耐受 

分 类 号：R392[医药卫生—免疫学]