白藜芦醇对体外培养的星形胶质细胞牵拉损伤后Glu和Gln释放的影响  

Effects of resveratrol on the release of Glu and Gln in cultured rat astrocytes with stretch injury

作　　者：周浩[1] 陈清[1] 郭江[1] 王茜[2] 孔丹莉[1] 俞守义[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广州510515 [2]南方医科大学南方医院,广州510515

出　　处：《南方医科大学学报》2008年第12期2215-2217,2220,共4页Journal of Southern Medical University

摘　　要：目的研究白藜芦醇对体外培养的星形胶质细胞牵拉损伤后氨基酸释放的影响;探索其对中枢神经保护作用的可能机制。方法采用不同剂量白藜芦醇与星形胶质细胞共同培养12h后,进行牵拉损伤,检测不同实验组培养基上清中的谷氨酸(Glu)和谷氨酰胺(Gln)含量,并进行乳酸脱氢酶(LDH)漏出量的测定。结果高效液相色谱结果显示,牵拉损伤使星形胶质细胞分泌Glu明显增加(P<0.05);1μmol白藜芦醇能够使星形胶质细胞损伤时Glu分泌进一步增加(P<0.05);而100μmol白藜芦醇能减少损伤时Glu分泌(P<0.05)。对细胞进行LDH漏出量的检测发现牵拉损伤能够使星形胶质细胞的LDH漏出量增加(P<0.05),1μmol白藜芦醇进一步加剧了LDH漏出(P<0.05),100μmol白藜芦醇能够有效减少星形胶质细胞的LDH漏出(P<0.05)。结论星形胶质细胞受到牵拉损伤后白藜芦醇能够减少其Glu,且能够减少LDH漏出,进而对星形胶质细胞起到保护作用。Objective To study the effects of resveratrol on amino acid release in cultured astrocytes with stretch injury and explore its possible mechanism for central nervous system protection. Methods Cultured rat astrocytes were treated with different concentrations of resveratrol for 12 h and the subsequent changes in Glu and Gin release and lactate dehydrogenase （LDH） leakage were examined after stretch injury. Results High-performance liquid chromatography showed that stretch injury increased Glu release from the astrocytes （P〈0.05）, and at the concentration of 1 μmol/L, resveratrol further increased Glu release （P〈0.05）, whereas at 100μmol/L, resveratrol obviously inhibit Glu release （P〈0.05）. LDH leakage increased significantly after stretch injury of the astrocytes （P〈0.05）, and resveratrol acted to further increase LDH leakage at 1 μmol/L but inhibited LDH leakage at 100 μmol/L （P〈0.05）. Conclusion Resveratrol can inhibit the release of Glu and LDH leakage from rat astrocytes to protect the cells from stretch injury.

关 键 词：白藜芦醇 星形胶质细胞 牵拉损伤 

分 类 号：R285.5[医药卫生—中药学]