青蒿琥酯对人子宫内膜癌细胞RL95-2的生长抑制作用  

Artesunate suppresses human endometrial carcinoma RL95-2 cell proliferation by inducing cell apoptosis

作　　者：郑军生[1] 王明华[2] 黄敏[1] 骆云鹏[3] 米粲[2] 

机构地区：[1]中山大学附属第三医院妇产科,广东广州510630 [2]重庆医科大学病理教研室,重庆400016 [3]重庆医科大学病理生理教研室,重庆400016

出　　处：《南方医科大学学报》2008年第12期2221-2223,共3页Journal of Southern Medical University

摘　　要：目的研究青蒿琥酯在体外对人子宫内膜癌RL95-2细胞的生长抑制作用及其可能机制。方法通过MTT法实验观察青蒿琥酯在体外对RL95-2细胞的抑制作用;透射电镜观察癌细胞的形态学改变;免疫组化检测细胞浆Caspase3活性;流式细胞术观察青蒿琥酯对细胞周期的影响及检测细胞线粒体膜电位Δψm和细胞内ROS水平。结果青蒿琥酯对RL95-2细胞具有增殖抑制作用,其半数细胞抑制浓度(IC50)为26.29μg/ml。透射电镜见:细胞呈早期凋亡改变:核染色质聚集于核膜周围,呈团块状;免疫组化检测细胞浆Caspase3表达阳性;流式细胞仪检测:细胞周期阻滞发生在G/0G1期;细胞内ROS升高(P<0.05);细胞线粒体膜电位Δψm降低(P<0.05)。结论青蒿琥酯在体外作用于人子宫颈癌RL95-2细胞,可能通过诱导细胞凋亡抑制癌细胞增殖。Objective To investigate the inhibitory effect of artesunate on human endometrial carcinoma RL95-2 cell line proliferation in vitro and the possible mechanisms. Methods The inhibitory effect of artesunate on the cell proliferation was assessed with MTT assay. Transmission electron miscrosopy was used to observe the morphological change of the cells after the treatment. Flow cytometry was performed to examine the changes in the cell cycle, reactive oxygen species （ROS） levels （with DCFH-DA labeling） and mitochondrial membrane potential （rhodamine123 staining）, and caspase-3 activity was detected by immunohistochemistry. Results Artesunate inhibited the proliferation of RL95-2 cells with an IC50 of 26.29 μg/ml. Transmission electron microscopy revealed early apoptotic changes of the cells with obvious chromatin fragmentation. The cell cycle arrest at G0/G1 phase was observed by flow cytometry, and immunohistochemistry demonstrated caspase-3 positivity in cytoplasm. ROS generation in the cells increased obviously after treatment with artestmate for 72 h, which also resulted in lowered mitochondrial membrane potential. Conclusions Artesunate suppressed the proliferation of RL95-2 cells in vitro possibly by inducing cell apoptosis.

关 键 词：青蒿琥酯 人子宫内膜癌细胞株RL95—2 氧自由基 线粒体膜电位 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R737.33[医药卫生—中医学]