花粉管通道法介导PRSV-CP基因dsRNA转化番木瓜  

Transformation of PRSV-CP dsRNA Gene into Papaya by Pollen-tube Pathway Technique

作　　者：魏军亚[1] 刘德兵[2] 陈业渊[1] 蔡群芳[3] 周鹏[3] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]海南大学园林园艺学院,海南儋州571737 [3]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《西北植物学报》2008年第11期2159-2163,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30560082);国家转基因植物研究与产业化开发专项(JY04-B-02);海南省自然基金项目(30823)

摘　　要：以番木瓜‘蔬罗Ⅰ号’植株为受体材料,采用花粉管通道技术将番木瓜环斑病毒外壳蛋白(PRSV-CP)基因3′-端同源序列dsRNA转入番木瓜子房中。用PCR方法对T0代种子进行了分子检测,获得53株转基因番木瓜,转化率达到8.9%;对获得的T0代转基因番木瓜进行了田间抗病性鉴定,结果表明,转基因番木瓜植株对番木瓜环斑病毒(PRSV)表现为不同程度的抗性,可以推迟发病。With papaya ‘Solo Ⅰ ＇ plants as reporters, the dsRNA-mediated 3＇-end homologous segment of PRSV-CP gene was transferred into flowers of receptors through pollen-tube pathway. Fifty-three positive transgenic papaya plants were confirmed by PCR assay among 1 200 plants and the transformation frequency was 8.9%. The resistance evaluation of T0 generation of the transgenic papaya plants carrying dsRNAmediated 3＇-end homologous segment of PRSV-CP gene were carried out. Compared with non-transgenic plants, the transgenic lines showed different degree resistance after virus infection and delayed symptom development.

关 键 词：花粉管通道 DSRNA 番木瓜环斑病毒(PRSV) 

分 类 号：Q789[生物学—分子生物学]