乙肝病毒基因免疫小鼠初步研究  

Constructs of Hepatitis B Virus Core DNAbased Vaccine and Immune Response in Balb/c Mice

作　　者：邓涛[1] 陈乃玲[1] 陈天宝[1] 武珊珊 张哓红 刘倩[1] 贾克明[1] 

机构地区：[1]中国人民解放军肝病研究所

出　　处：《中国公共卫生学报》1998年第1期13-15,共3页

基　　金：国家自然科学基金

摘　　要：将经过基因修饰的乙肝病毒核心区基因定向克隆于质粒ｐｃＤＮＡ３巨细胞病毒启动子下游，构建成真核表达载体，称为ｐｃＤＮＡ３－ＨＢＶ。将ｐｃＤＮＡ３－ＨＢＶ通过多点肌肉注射，给ＢＡＬＢ／ｃ小鼠免疫接种１００μｇ／只，２周后追加免疫１次，追加免疫１周后８只免疫鼠中７只出现抗－ＨＢｃＩｇＧ血清转化，而接种空载质粒ｐｃＤＮＡ３的对照组及未经任何免疫接种的ＢＡＬＢ／ｃ小鼠无一只抗－ＨＢｃＩｇＧ阳性。The modificated HBc gene with its stem and loopstructure trancated was inserted into the eukaryotic expression vector under the CMV promotor between the Hind Ⅲ and Bam HI site (pcDNA3HBV).The Balb/c mice were immunized by multiple sites intramuscular injection with pcDNA3HBV and pcDNA3 respectively.Each mouse was injected with 100μg DNA(1μg/μl in PBS).Another 100μg DNA were reinjected after 2 weeks.The antiHBc IgG were detected by ELISA in 7 out of 8 immunized mice (pcDNA3HBV group)after 7 days,but no antiHBc IgG Ab could be measured in pcDNA3 immunized group(n=4) and nonimmunized group(n=4)

关 键 词：乙肝病毒 DNA 基因免疫 乙型肝炎 

分 类 号：R512.620.5[医药卫生—内科学]