大黄素抗大鼠肝移植急性排斥反应的实验研究  

Empirical study on effect of emodin on acute rejection after orthotopic liver transplantation in rats

作　　者：童洪飞[1] 林胜璋[1] 杨潇[1] 荆河[1] 

机构地区：[1]温州医学院附属第二医院,温州325027

出　　处：《中华中医药杂志》2009年第1期49-52,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30572395)

摘　　要：目的:探讨大黄素对大鼠肝移植术后急性排斥反应的抑制作用。方法:建立LEW→BN大鼠肝移植动物模型,随机分为A组(排斥反应组)、B组(CsA组)、C组(大黄素组)3组,每组15只大鼠,术后每天分别腹腔注射生理盐水0.5ml/d、环孢素A(CsA)10.0mg·kg-1·d-1、大黄素50.0mg·kg-1·d-1,连续用药8天,停药后每组处死6只大鼠取肝脏组织观察急性排斥活动指数(RAI)和肝细胞凋亡指数(AI),其余大鼠继续饲养以观察存活期。结果:A、B、C组大鼠存活期(天)分别为9.50±1.64、21.57±2.15、21.29±2.21,B、C组存活期较A组明显延长(P<0.01),B、C组间存活期无明显差别(P>0.05)。A、B、C组RAI分别是7.67±0.98、5.17±0.40、5.83±0.75,AI分别是35.83±2.32、15.83±1.33、16.50±2.35。B、C组的RAI、AI较A组均明显降低(P<0.01),B、C组间差别均无意义(P>0.05)。结论:大黄素能有效减少大鼠肝移植术后肝细胞的凋亡,抑制肝移植术后急性排斥反应。Objective： To evaluate the effect of emodin on acute rejection after orthotopic liver transplantation （OLT） in rats. Methods ： The LEW→BN OLT models were established. A total of 45 rats were divided randomly and equally into 3 groups： group A was treated with normal saline at dose of 0.5 ml/d intraperitoneally from 1st day to 8th day after operation; Group B, CsA at dose of 10.0 mg·kg^-1·d^-1 ; Group C, emodin at dose of 50.0 mg·kg^-1·d^-1. 8 days after operation, 6 recipients of each groups were killed for confirming rejection-active index （RAI） and hepatocellular apoptosis index （AI） by observing the pathologic change of transplanted liver in recipients. The other recipients were raised for observing the survival time. Results： Respectively, the survival time（days） of group A,B,C was 9.50±1.64,21.57±2.15,21.29±2.21. The survival time of group B,C was significantly longer than that of group A （ P〈0.01 ） and there was no significant distinction between group B and C in survival time （P〉0.05）. Respectively, the RAI of group A ,B,C was 7.67±0.9,5.17±0.40,5.83±0.75 and the AI of group A,B,C was 35.83±2.32,15.83±1.33,16.50±2.35. The RAI and AI of group B,C was significantly lower than that of group A （ P〈0.01 ） and there was no significant distinction between group B and C in RAI and AI （ P〉0.05 ） . Conclusion ： Emodin can reduce the hepatocellular apoptosis and suppress the acute rejection after OLT in rats.

关 键 词：大黄素 肝移植 急性排斥 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]