加减抵挡汤对肾小球硬化大鼠TIMP-1及PAI-1mRNA表达的影响  

Effect of modified Didang Decoction on expression of TIMP-1 and PAI-1mRNA in glomerulosclerosis rats

作　　者：柴可夫[1] 张曾亮[1] 黄晓玲[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《中华中医药杂志》2009年第1期93-95,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药科研基金计划(No.2002C001)

摘　　要：目的:探讨加减抵挡汤治疗肾小球硬化的机制。方法:采用单侧肾切除重复阿霉素注射造成肾小球硬化大鼠模型,以加减抵挡汤治疗并用西药科素亚为阳性对照,观察模型组和各治疗组的肾功能及24h尿蛋白定量,采用逆转录多聚酶链反应(RT-PCR)测定各组大鼠肾组织TIMP-1及其PAI-1的mRNA的表达。结果:加减抵挡汤各组均能降低肾小球硬化大鼠增高的24h尿蛋白,改善肾功能,且加减抵挡汤能下调大鼠肾组织TIMP-1和PAI-1mRNA的表达。结论:加减抵挡汤通过下调肾小球硬化大鼠肾组织TIMP-1和PAI-1 mRNA的表达,延缓肾功能衰竭。Objective： To discuss the influence of Didang Decoction on glomerulosclerosis. Methods： The glomemlosclerosis model was established by uninephrectom and injection of adriamyci, and was treated by modified Didang Decoction and taken western medicine Losartan as the comparison group. To observe 24-hour urinary protein and kidney function of model group and each treatment group. Reverse transcriptase Polymerase Chain Reaction（RT-PCR） was used to determine the expression of TIMP-1 and PAI-1 in each group of rats kidney tissue. Results： Didang Decoction could decline elevated 24-hour urinary protein and improve kidney function, and decline the expression of TIMP-1 and PAI-lmRNA in glomerulosclerosis rats. Conclusion： Didang decoction could delay renal failure, it may be related to the down-regulated expression of TIMP-1 and PAI-1mRNA.

关 键 词：抵挡汤 肾小球硬化 纤溶酶原激活物抑制物 金属蛋白酶组织抑制因子 

分 类 号：R285.5[医药卫生—中药学] R575.2[医药卫生—中医学]