家兔尿中SFZ-47及其两种主要代谢产物的HPLC定量方法  

HPLC Determination of 3H-1,2-Dihydro-2-(4-methyophenylamino) Methyl-1-Pyrrolizinone and Its Two Major Metabolites in Rabbit Urine

作　　者：顾景凯[1] 钟大放[1] 翟志慧 

机构地区：[1]沈阳药科大学药学系,沈阳110015

出　　处：《沈阳药科大学学报》1998年第1期13-17,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金

摘　　要：建立了家兔尿中ＳＦＺ－４７及其两种主要代谢产物ＳＦＺ－４７羧基衍生物（Ｍ１）及该衍生物的酯型β－Ｄ－葡糖苷酸（Ｍ２）的 ＨＰＬＣ测定方法，并用该法对单剂量口服２３０ｍｇ ＳＦＺ－４７的 ３只家兔尿样进行了定量分析．结果表明：在０～２４ｈ内，ＳＦＺ－４７，ＭＩ和Ｍ２的累积排泄量分别占给药剂量的（８．３±０．４８）％，（１８．９３±２．６６）％和（２３．４２±８．００）％．The major metabolites of 3H-l,2-dihydro-2-(4-methylphenylamino)methyl-1-pyrro- lizinone(SFZ-47) in rabbits, a new anti-inflammatory and analgesic agent are 4-(3H-1,2-dihy- dro-1-pyrrolizinone-2-methylamino) benzoic acid(M1) and its acyl β-D-glucuronide(M2).A HPLC method for the quantitative determination of SFZ-47 and its two major metabolites in rabbit urine was developed in terms of specificity,precision,and accuracy,and was found to be reliable and reproducible.In three rabbits after a 230 mg p.o. dose of SFZ-47,the analytical results showed that the excretion rate of the parent drug was (8.90±0.48)% of the total dose administrated in 24 h,while those of M1 and M2 were (18.93±2.66)% and (23.42±8 .00) %, respectively.

关 键 词：SFZ-47 抗炎镇痛剂 代谢物 高效液相色谱法 

分 类 号：R971.1[医药卫生—药品] R914.5[医药卫生—药学]