巨噬细胞条件培养基影响乳鼠雪旺细胞分泌神经生长因子的实验研究  

The effect of the macrophages conditioned media(MOCM) on NGF secretion in newborn rat schwann cells in vitro

作　　者：马向阳[1,2,2] 于涯涛[1,2,2] 钟世镇[1,2,2] 胡耀民 陈立龙[1,2,2] 

机构地区：[1]第一军医大学解剖学教研室 [2]解放军第一五七医院骨科

出　　处：《中华外科杂志》1998年第2期80-82,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金

摘　　要：目的探讨巨噬细胞对雪旺细胞分泌神经生长因子（ＮＧＦ）的影响。方法制备脂多糖激活的和未激活的成年大鼠腹腔巨噬细胞的条件培养基，取３种不同浓度，分别施加于纯化培养的乳鼠雪旺细胞，作用２４、４８、７２小时，取细胞上清液，用间接ＥＬＩＳＡ法测定细胞上清液中ＮＧＦ的含量。结果脂多糖激活的巨噬细胞条件培养基和未激活者取不同浓度作用不同时间后均能不同程度的促进乳鼠雪旺细胞分泌ＮＧＦ。结论实验结果表明，巨噬细胞条件培养基中含有促进雪旺细胞分泌ＮＧＦ的因子，提示巨噬细胞可能通过分泌细胞因子调节雪旺细胞对ＮＧＦ的分泌。Objective To study the influence of macrophages on nerve growth factor (NGF) secretion in newborn rat schwann cells.Methods LPS activated and not activated adult rat peritoneal macrophages conditioned media (MOCM) were treated on purified cultured Schwann cells for 24,48 and 72 hours in three different concentrations the NGF contents in the cultured Schwann cells suspension media were measured by a two site enzyme linked immunoserbent assay.Results Both L MOCM (LPS activated MOCM) and N MOCM(not activated MOCM) obviously enhanced NGF secretion in chwann cells in each different concentration or testing time.Conclusion MOCM contains some factors which can promote NGF secretion in rat schwann cells in vitro, suggesting that macrophages regulating NGF secretion by secreted certain cytokines.

关 键 词：神经生长因子 巨噬细胞 雪旺细胞 神经损伤 

分 类 号：R651.302[医药卫生—外科学] R362[医药卫生—临床医学]