TWEAK诱导类风湿关节炎成纤维样滑膜细胞合成MMP-3的实验研究  

Effects of TWEAK on the synthesis of MMP-3 in fibroblast-like synoviocytes of rheumatoid arthritis

作　　者：夏丽萍[1] 肖卫国[1] 李俊松[1] 丁爽[1] 鲁静[1] 

机构地区：[1]中国医科大学附属第一医院风湿免疫科,辽宁沈阳110001

出　　处：《细胞与分子免疫学杂志》2009年第1期46-48,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：辽宁省教育厅高等学校科学研究资金资助项目(202013141);辽宁省自然科学基金资助项目(20042092)

摘　　要：目的:通过观察TWEAK在不同浓度下对RAFLS诱导合成MMP-3的影响,探讨TWEAK参与类风湿关节炎(RA)关节骨及软骨破坏的相关机制,进而寻求治疗RA的新途径。方法:将rhTWEAK与成纤维样滑膜细胞(FLS)共培养,应用ELISA法检测细胞培养液中MMP-3水平;用反转录-聚合酶反应(RT-PCR)检测FLS中MMP-3mRNA的表达水平。结果:TWEAK终浓度为50、100μg/L组诱导RAFLS合成MMP-3的水平明显高于对照组,具有显著的统计学意义(P<0.05);TWEAK终浓度为100μg/L诱导RAFLS的MMP-3mRNA表达水平为对照组的1.26倍,具有显著的统计学意义(P<0.05);TNF-α和IL-1β对TWEAK诱导RAFLS合成MMP-3及MMP-3mRNA表达具有协同作用,协同作用组MMP-3的水平及MMP-3mRNA表达水平明显高于单纯TWEAK组,具有显著的统计学意义(P<0.05)。结论:TWEAK通过诱导RAFLS合成MMP-3,直接参与RA的关节损伤,TNF-α和IL-1β在此过程中发挥协同作用。AIM： To investigate the effects of TWEAK on the synthesis of MMP-3 in RA FLS at different concentrations and to discuss the relative mechanism of how TWEAK involves in the destruction of articular bone and cartilage. METHODS： RA FLS were primarily cultured and stimulated with TWEAK. ELISA was used to detect the concentration of MMP-3 in cell-cultured fluid. The gene mRNA expression of MMP-3 was measured by RT-PCR. RESULTS： The level of MMP-3 induced by TWEAK at 50 and 100 μg/L was higher than that in control group, which had significant statistic difference（ P 〈 0.05）. The expression level of MMP-3mRNA induced by TWEAK at 100 μg/L was 1.26 times higher than that in the control group, which had significant statistic difference （ P 〈 0.05 ）. TNF-α and IL-1β had synergetic effect on the synthesis and mRNA expression of MMP-3. The level in the synergetic group was significantly higher than that in the simple TWEAK group , which had significant statistic difference（ P 〈 0.05）. CONCLUSION： TWEAK can induce RA FLS to synthesize MMP-3 and damage the articular bone and cartilage directly. TNF-α, IL-1β and TWEAK had synergetic effects during the synthesis of MMP-3 in RA FLS.

关 键 词：关节炎 类风湿 TWEAK 成纤维样滑膜细胞 基质金属蛋白酶3 

分 类 号：R392.11[医药卫生—免疫学]