鞘内注射烟碱对异氟烷、七氟烷镇痛作用的影响  

EFFECTS OF INTRATHECAL INJECTION OF NICOTINE ON THE ANALGESIC ACTIONS OF ISOFLURANE AND SEVOFLURANE

作　　者：闫肃[1] 戴体俊[1] 曾因明[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,徐州221002

出　　处：《中国疼痛医学杂志》2008年第6期352-355,共4页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助项目(30471657;39970715);江苏省自然科学基金资助项目(BK2001143)

摘　　要：目的:探讨脊髓神经元烟碱受体(neuronal nicotinic acetylcholine receptors,nnAChRs)与异氟烷、七氟烷镇痛作用的关系。方法:给小鼠腹腔注射异氟烷、七氟烷后,用热板法和福尔马林法分别观察鞘内(intrathecal,it)注射不同剂量的烟碱(nicotine,N)对小鼠热板法痛阈(pain threshold in hot-plate test,HPPT)和Ⅰ相、Ⅱ相舔被注射足累积时间(paw licking time,PLT)的影响。结果:热板法实验中,烟碱5、10、15μgit对清醒小鼠HPPT没有影响(P>0.05),但能够剂量依赖性地减少异氟烷、七氟烷镇痛小鼠的HPPT(P<0.05,P<0.01);福尔马林法实验中,腹腔注射镇痛剂量的异氟烷、七氟烷后均能引起小鼠I相和II相PLT减少(P<0.01),烟碱5、10、15μgit对异氟烷、七氟烷镇痛小鼠Ⅰ相和Ⅱ相PLT影响的差异均无显著性(P>0.05)。结论:nnAChRs是异氟烷、七氟烷抗热刺激伤害的重要靶位,但与其抗化学炎性痛作用关系不大。Objective：To investigate the relationship between spinal cord neuronal nicotinic acetylcholine receptors（nnAChRs） and the analgesic effects of isoflurane and sevoflurane. Methods ：After the mice was established model of analgesia by intraperitoneally injecting （ip） appropriate doses of isoflurane or sevoflurane, different doses of nicotine were injected intrathecally. The pain threshold in hot-plate test （HPFF） using hot-plate test and phase Ⅰ and Ⅱ paw licking time （PLT） using formalin test were obseved. Results：In hot-plate test, nicotine with 5, 10, 15 μg （it） did not affect the HPPT in conscious mice （P 〉0.05）. By contrast, nicotine with 5, 10, 15 μg （it） dose-dependently decreased the HPPT of the mice treated with isoflurane or sevoflurane （P 〈0.05 or P 〈0.01 ） ;In formalin test, phase Ⅰ and Ⅱ paw licking time inhibition induced by isoflurane or sevoflurane was not affected by intrathecally injected nicotine. Conclusion： nnAChRs may be important targets for the analgesic effects of isoflurane and sevoflurane on thermal-induced nociception but not on chemical and inflammatory-induced nociception.

关 键 词：神经元烟碱受体 异氟烷 七氟烷 镇痛 

分 类 号：R96[医药卫生—药理学]