中药复方海康灵含药血清对过氧化氢诱导的神经细胞凋亡的影响  

Effects of Haikangling in Serum on Cultured Neuroblastoma Apoptosis Induced by Hydrogen Peroxide

作　　者：孙向红[1,2] 孙伟[1] 李静[2] 耿美玉[2] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]中国海洋大学医药学院,山东青岛266003

出　　处：《中国药学杂志》2008年第23期1781-1784,共4页Chinese Pharmaceutical Journal

摘　　要：目的观察海康灵对SH-SY5Y神经细胞凋亡、细胞内线粒体膜电位和钙离子的影响。方法采用血清药理学方法,用流式细胞仪观察海康灵对过氧化氢(H2O2)损伤的SH-SY5Y神经细胞凋亡、细胞线粒体膜电位和钙离子的影响。结果与模型组比较,海康灵含药血清能明显抑制H2O2引起的SH-SY5Y神经细胞的凋亡,稳定线粒体膜电位,抑制钙离子内流。结论以上结果提示,海康灵可通过抑制胞浆钙离子升高,进而稳定线粒体膜电位,从而发挥其抑制神经细胞凋亡的作用。OBJECTIVE To observe the effect of Haikangling in serum on apoptosis, mitochondria membrane potential and Ca^2＋ of neuroblastoma SH-SY5Y ceils. METHODS The effects of Haikangling in serum on apoptosis, mitochondria membrane potential and Ca^2＋ of SH-SYSY cells impaired by hydogen peroxide （H2O2 ）cultured in vitro were measured by flow cytometry. RESULTS Compared with the model group, Haikangling obviously restrained the apoptosis, Ca^2＋ inflow, and stabilized the mitochondria membrane potential of SH-SY5Y cells. CONCLUSION Anti-dementia effects of Haikangling were related to restraining the apoptosis, stabilizing mitochondria membrane potential and restraining Ca^2＋ inflow of neurocyte.

关 键 词：海康灵 过氧化氢 凋亡 线粒体膜电位 钙离子 

分 类 号：R965[医药卫生—药理学]