羟乙葛根素对大鼠局灶性脑缺血再灌注损伤后脑组织E-selectin及P-selectin表达的影响  

Effect of Hydroxyethylpuerarin on Expression of E-selectin and P-selectin in Rats with Focal Cerebral Ischemia-Reperfusion Injury

作　　者：娄海燕[1] 魏欣冰[1] 刘慧青[1] 王姿颖[1] 张岫美[1] 

机构地区：[1]山东大学医学院药理学研究所,济南250012

出　　处：《中国药学杂志》2008年第23期1788-1791,共4页Chinese Pharmaceutical Journal

摘　　要：目的观察E-selectin及P-selectin在羟乙葛根素保护大鼠局灶性脑缺血再灌注损伤中的作用。方法线栓法制备大鼠局灶性脑缺血再灌注损伤模型,大鼠脑缺血2 h再灌注24 h后,采用Western blot及RT-PCR法观察大鼠脑组织E-selectin及P-selectin的蛋白及mRNA表达情况。结果羟乙葛根素可明显降低缺血区脑组织E-selectin及P-selectin的蛋白及mRNA表达。结论羟乙葛根素可能通过降低缺血区脑组织黏附分子表达发挥脑保护作用。OBJECTIVE To observe the effects of hydroxyethylpuerarin on expression of E-selectin and P-selectin in rats with focal cerebral ischemia-reperfusion injury and explore its action mechanism. METHODS Rats were subjected to cerebral ischemiareperfusion injury by middle cerebral artery occlusion （MCA0）. After a 2 h ischemia and 24 h reperfusion, the protein and mRNA expression of E-selectin and P-selectin were evaluated by western blot and RT-PCR analysis. RESULTS Hydroxyethylpuerarin significantly reduced the expression of E-selectin and P-selectin in ischemic brain tissue induced by cerebral ischemia-reperfusion. CONCLUSION Hydroxyethylpuerarin may attenuate brain ischemia injury by down-regulating the expression of adhesion molecules in ischemic brain tissues.

关 键 词：羟乙葛根素 脑缺血再灌注损伤 E-SELECTIN P-SELECTIN 

分 类 号：R965[医药卫生—药理学]