FSH对绵羊卵母细胞体外核成熟的影响  

Effect of FSH on Nuclear Maturation of Sheep Oocyte Matured in Vitro

作　　者：曲杨燕[1] 赵雁伟[1] 李凯[1] 刘云海[1] 郭勇[1] 倪和民[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国畜牧兽医》2008年第12期80-83,共4页China Animal Husbandry & Veterinary Medicine

基　　金：北京市教育委员会科技发展计划重点项目(KZ200510020013);北京市教育委员会科技发展计划面上项目(KM200710020006);2006年度人事部留学人员科研活动择优资助项目(启动类)

摘　　要：为了探讨FSH对绵羊卵母细胞核成熟的影响,本试验将绵羊卵母细胞体外成熟24 h,并在成熟过程中的4个不同时间段内添加FSH,统计各个时间段卵母细胞的生发泡破裂（germinal vesicle break down,GVBD）及第一极体排出情况。结果显示：①在体外成熟培养的前4 h或前8 h添加FSH,经体外成熟的卵母细胞其生发泡破裂率与不添加FSH组无显著差异;②在体外成熟的4-24 h或8-24 h添加FSH,其第一极体排出率与不添加FSH组有极显著差异。说明,在本试验条件下,FSH对绵羊卵母细胞体外成熟具有显著的促进作用,是在减数分裂的恢复后到减数分裂完成之间的某一阶段起的促进作用。To investigate the effect of FSH on nuclear maturation of sheep oocyte matured in vitro, the COC were cultured in IVM medium for 24 h, and were divided into 4 tgroups according to four different durations. Then, the rates of GVBD and exclusion of polar body Ⅰ （Pb Ⅰ）were also analyzed respectively. Results： there were no significant differences in rate of GVBD among those three groups treated with FSH in the first 4 h or 8 h,but there were significant differences in rate of exclusion of Pb I of oocytes among the groups added with FSH from 4 h to 24 h,or 8 h to 24 h. Conclusion： FSH does not promote the meiotie division of ovine oocytes matured in vitro during the first 8 h, but it may play an important role during the period between the stage of GVBD and exclusion of Pb Ⅰ.

关 键 词：绵羊 卵母细胞 FSH 核成熟 体外成熟 

分 类 号：S814.8[农业科学—畜牧学]