大马勃水溶性多糖的结构研究  

Isolation,Purification and Structural Investigation of Polysaccharide from Calvatia geigantea

作　　者：武翠玲[1,2] 邓永康[2] 孟延发[2] 

机构地区：[1]长治医学院心血管研究所,长治046000 [2]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065

出　　处：《天然产物研究与开发》2008年第6期1027-1030,共4页Natural Product Research and Development

摘　　要：采用热水浸提,乙醇沉淀的方法得到大马勃粗多糖,经Sevag法脱蛋白,DEAE-sepharose fast flow离子交换层析及sephacryl S-300HR凝胶过滤法得大马勃多糖(CGPⅠ-1)。气相色谱研究表明,其单糖组成为:Gal,Glc,Man等四种单糖,其中Gal,Glc,Man,摩尔比为3.92∶11.28∶1.22。经部分酸水解,红外光谱及核磁共振光谱,高碘酸氧化,Smith降解等分析,CGPⅠ-1的主链由Man和Glc构成,存在β型和α型两种糖苷键构型,支链或主链的末端残基由β-Gal(1→4),β-Glc(1→6),α-Glc(1→4)构成,其分子中存在酰胺结构。原子力显微镜观察发现CGPⅠ-1呈分支的线性分子,在水溶液中容易互相缠绕形成强大的网络结构。Calvatia geigantea polysaccharide CGP was extracted by hot water,precipitated by ethanol, and deproteinized by Sevag method. The CGP I -1, a main constituent of CGP, was obtained by DEAE-sepharose and Sephacryl S-300HR gel-chromatography. The acid hydrolyzed product and GC-MS analysis of the CGP I -1 showed that CGP I -1 is composed of Gal, Glc, Man and so on. Gal, Glc and Man in a molar ratio of 3.9： I 1.28： 1.22. IR analysis, partial hydrolysis with acid,periodate oxidation, Smith degradation, and ^1H NMR, ^13C NMR analysis revealed that the main chain of CGP I -1 is mainly made up of Man and Glc. The side chain or terminal residues is composed of β-Gal（1→4） ,β-Glc（ 1→6） ,α-Glc（ 1→4）. There was amide group in CGP I -1. The molecular structure of the CGP I -1 at different concentrations was observed with atomic force microscopy（AFM）. The CGP I -1 molecule had a linear backbone with branches. The net work structure of CGP I -1 was formed through entanglement between the molecules of the polysaccharide.

关 键 词：大马勃 多糖 分离纯化 结构分析 

分 类 号：Q50[生物学—生物化学] R284.1[医药卫生—中药学]