牵张作用对成骨样细胞MG63的线粒体跨膜电位的影响  

Stretching-induced the mitochondrial membrane potential and apoptosis in human osteoblast-like MG63

作　　者：张卫群[1] 刘云启[2] 朱智敏[3] 巢永烈[3] 

机构地区：[1]山东大学附属省立医院口腔种植科,山东济南250021 [2]滨州医学院附属医院身肾内科,山东滨州256600 [3]四川大学华西口腔医学院,四川成都610041

出　　处：《现代生物医学进展》2008年第12期2269-2271,2274,共4页Progress in Modern Biomedicine

基　　金：滨州医学院科技攻关项目(2006-7 BY2005KJ19)

摘　　要：目的:探讨在低渗透压形成的静牵张应力环境下线粒体跨膜电位与细胞增殖分化及凋亡的关系。方法:用成骨样细胞MG63细胞株进行体外培养、扩增,在对数生长期采用不同的低渗透压对细胞进行刺激,检测不同作用务件下线粒体跨膜电位(ΔΨm)、细胞增殖比例(S期百分比)以及凋亡指数。结果:240mOsm组ΔΨm呈上升趋势,4h达到峰值,6h逐渐下降,但仍高于对照组;163 mOsm组ΔΨm在6 h时明显降低。277和240 mOsm组S期百分比在6 h和8 h达到峰值(26.54±0.71,28.10±0.39:26.96±0.33,28.55±0.26)。三个实验组的凋亡峰均提前,且大于对照组,尤以163 mOsm组为最(54.87±0.78★)。结论:成骨样细胞MG63ΔΨm的变化与时间和力学刺激强度有一定的依赖性,预示线粒体跨膜电位的变化与细胞增殖活性之间存在一定的关系。Objective： To explore the relationships between mitochondrial membrane potential and proliferation index or apoptosis radio by stretching of hyposmolality solutions. Methods： Mechanical loading was applied by swelling osteoblast-like cells MG63 in hypotonic solutions. Scanning Laser Confocal Microscope imaging of mitochondria stained with JC-1, a trans-membrane potential-sensi- tive vital dye, showed that ratio of red/green, mitochondrial membrane potential. Cell apoptosis ratio and proliferation index were used to assay apoptosis and proliferation of osteoblast-like cell MG63. Results： Mitochondria membrane potential （ΔΨm） increased following the accrescenee of swelling. The ΔΨm was highest under 240 mOsm at 4 h, while the lowest under 163 mOsm at 6 h. The proliferation index of four groups showed maximum at 6 h and 8 h （26.54± 0.71, 28.10± 0.39; 26.96± 0.33, 28.55± 0.26）. With the decreasing ofhypotonic pressure, apopotosis ratio increased, the 163mOsm group showed higher （24.68 ± 0.51）. Conclusions： The response of MG63 osteoblast-like cell line was dependent on the magnitude and time of the swelling applied, which showed a tight relationship between the mitochondrial membrane potential and proliferation and apoptosis in MG63 osteoblast-like cell line.

关 键 词：成骨样细胞 牵张作用 线粒体跨膜电位 凋亡 

分 类 号：R246.83[医药卫生—针灸推拿学]