西北地区沙漠尘对大鼠肺巨噬细胞毒性的实验研究  

Toxic Effect of Desert Dust of Northwest Areas of China on Alveolar Macrophages of Rats

作　　者：高金霞[1] 王振全[1] 王式功[2] 连素琴[1] 常旭红[1] 严俊[1] 王立辉[1] 

机构地区：[1]兰州大学公共卫生学院劳动卫生与环境卫生研究所,甘肃兰州730000 [2]兰州大学大气科学学院,甘肃兰州730000

出　　处：《环境与健康杂志》2008年第12期1056-1058,共3页Journal of Environment and Health

基　　金：干旱气象科学研究基金资助项目(IAM200703);兰州大学医学基金资助项目(lzuyx200624);<联合国防治荒漠化公约>的支撑技术研究与示范(2007BAC03A10)

摘　　要：目的探讨和田沙漠尘和民勤沙漠尘对大鼠肺巨噬细胞的毒性作用。方法用50、100、200、400、800、1600μg/ml的和田沙漠尘、民勤沙漠尘和SiO_2的混悬液培养肺巨噬细胞,用DMEM培养液培养肺巨噬细胞作为对照组,24 h后用MTT法测定肺巨噬细胞的存活率,并收集细胞及上清液,检测肺巨噬细胞内丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活力,上清液中LDH活力和转化生长因子(TGF-β_1)含量。结果与对照组比较,和田沙漠尘400μg/ml以上组、民勤沙漠尘100μg/ml以上组和SiO_2组的大鼠肺巨噬细胞存活率降低(P<0.05,P<0.01);随染毒剂量增加,大鼠肺巨噬细胞内MDA含量、GSH-Px活力,上清液中LDH活力、TGF-β_1含量均有增高趋势,和田沙漠尘和民勤沙漠尘浓度200μg/ml以上组MDA含量和GSH-Px活力高于对照组(P<0.05,P<0.01),和田和民勤沙漠尘浓度100μg/ml以上组和SiO_2组LDH活力高于对照组(P<0.01),两地沙漠尘浓度200μg/ml以上组和SiO_2组TGF-β_1含量高于对照组(P<0.01)。结论民勤沙漠尘和和田沙漠尘对肺巨噬细胞有一定的损伤作用,使肺巨噬细胞脂质过氧化作用增强,并能引起纤维化因子FGF-β_1的释放。Objective To explore the damage on alveolar macrophages （AMs） induced by desert dust of Hetian and Minqin, China. Methods AMs were cultured with different concentrations of desert dust and SiO2 respectively. AMs of the control group were cultured with normal DMEM culture medium. After 24 hours exposure, cells supernatant of culture media was collected and MDA content, GSH-Px activity, LDH activity and TGF-β1 content was measured. Results Compared with the control group, survival rate of AMs decreased significantly in 400, 800, 1 600 μg/ml desert dust groups of Hetian and 100, 200, 400, 800, 1 600μg/ml groups of Minqin, the content of MDA, GSH-Px activity and TGF-β1 content in 200 and 400 μg/ml desert dust groups of Hetian and Minqin increased significantly （P〈0.05）, while the activity of LDH in 100, 200 and 400 μg/ml groups of desert dust and SiO2 group increased significant （P〈0.01）. Conclusion Desert dust from Hetian and Minqin has cytotoxicity to AMs, can enhance lipid peroxidation and releases fibrosis factor TGF-β1.

关 键 词：空气污染 沙漠尘 肺巨噬细胞 

分 类 号：R122.2[医药卫生—环境卫生学]