2，4-二氯苯氧乙酸(2，4-D)对雨生红球藻中虾青素积累的影响  

The Impact of Extraneous Salicylic Acid (SA) to Astaxanthin Accumulation of Haematoccus pluvialis

作　　者：孟春晓[1] 高政权[1] 叶乃好[2] 

机构地区：[1]山东理工大学生命科学学院,淄博255049 [2]中国水产科学院黄海水产研究所,青岛266071

出　　处：《微生物学通报》2008年第12期1884-1887,共4页Microbiology China

基　　金：国家自然科学基金(No.40706050,No.40706048);国家支撑项目(No.11200602);中央级公益性研究所专项资金(No.2060402/2);山东理工大学自然科学基金(No.4040-306017);山东理工大学博士启动基金项目(No.4041-405016,4041-405017)

摘　　要：本文初步研究了一定浓度范围内的2,4-D对雨生红球藻积累虾青素的影响。在对数生长期的藻液中分别加入一系列不同浓度的2,4-D溶液,然后进行胁迫培养(25°C、24h、5000Lx连续光照+营养盐饥饿),诱导细胞内虾青素的合成积累。在诱导过程中,显微观察不同浓度2,4-D处理后细胞形态和虾青素积累的动态变化,并定期取样测定虾青素含量。结果表明,20.0mg/L的2,4-D能够明显促进雨生红球藻中积累虾青素。它不仅可以加快虾青素积累进程(比对照提前15d),而且比对照能提高13.4%的虾青素产量。Effect of extraneous 2,4-D on astaxanthin accumulation in H. pluvialis was studied in this paper. After different concentration grades of 2,4-D were added into the vegetative green cells respectively, then H. pluvialis were cultivated under unfavorable growth conditions （24 h light cycle, 5000 Lx, 25℃, nutrition deficiency） to promote astaxanthin accumulation. In this stage, morphological changes of cells treated by different grades of 2,4-D concentration were observed with optical microscopy. Moreover, astaxanthin content was determined with absorption spectral （00490）. Results showed that astaxanthin accumulation might be promoted at 20 mg/L 2,4-D treatment which could quicken astaxanthin accumulation 15 days compared with blank controls. Moreover, the increasing production extents of astaxanthin reached 13.4% than blank controis.

关 键 词：雨生红球藻 虾青素积累 2  4-二氯苯氧乙酸(2  4-D) 

分 类 号：Q943.1[生物学—植物学]