抗菌肽MagaininⅡ基因的克隆及其在E.coli BLP中的融合表达  

Cloning and Fusion Expression of Antibacterial Peptide MagaininⅡ in E.coli BLP

作　　者：陈晓平[1] 詹冬玲[1] 贾惠文[1] 商雪娇[1] 钱爱东[2] 

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118 [2]吉林农业大学动物科技学院,长春130118

出　　处：《吉林农业大学学报》2008年第6期797-800,804,共5页Journal of Jilin Agricultural University

基　　金：吉林省科技攻关项目(20060208)

摘　　要：采用SOE法人工设计和合成抗菌肽MagaininⅡ基因,定向克隆至表达载体pET 28a,将构建的重组质粒pET 28a-Mag转化至E.coliBLP,Amp抗性筛选的阳性克隆用IPTG诱导融合表达。利用Tricine-SDS-PAGE确定了产物的正确性,利用琼脂孔穴扩散法证明表达产物具有抗菌活性。The antibacterial peptide Magainin Ⅱ gene was designed and artificially synthesized by SOE. Antibacterial peptide Magainin Ⅱ gene was cloned into eukaryotic expression vector pET 28a, pET 28a- Mag transformed into E. coli BLP. The positive clones were identified by Amp. They were induced by IPTG and the fusion expression product was examined by Tricine-SDS-PAGE. Results of agarose diffusion assay indicated that the expression product exhibited the antibacterial activity.

关 键 词：抗菌肽MagaininⅡ SOE法 合成 克隆 融合表达 

分 类 号：Q78[生物学—分子生物学]