青龙衣中胡桃醌对A-549细胞周期及凋亡影响  

Effect of juglone in Qinglongyi on cell cycle status and apoptosis in A-549 cells

作　　者：孔陵生[1,2] 邹翔[1,2] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2008年第6期641-644,677,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(30600816);高等学校博士点学科点专项科研基金(20060240001)

摘　　要：探讨青龙衣中胡桃醌(Juglone)对体外培养的人肺癌细胞A-549生长抑制作用及对细胞凋亡和周期的影响.采用MTT法检测青龙衣中胡桃醌对A-549细胞的生长抑制作用,采用激光共聚焦扫描显微镜观察凋亡形态,流式细胞仪分析胡桃醌对A-549细胞周期的影响.MTT法试验结果显示,胡桃醌对体外培养的人肺癌细胞A-549具有明显的生长抑制作用,处理24、48、72 h的IC50值为分别为3.4×10-5、1.8×10-5、2.6×10-6mol/L.凋亡形态观察结果显示,胡桃醌能诱导A-549细胞的凋亡,同时胡桃醌能剂量依赖性的降低A-549细胞的G1期细胞的比例,升高G2期和S期细胞比例,呈G2期和S期阻滞作用.胡桃醌对A-549细胞具有明显的生长抑制作用,阻止周期进程,诱导A-549细胞凋亡.To explore the inhibition of juglone in Qinglongyi on A -549 cells in vitro, MTr assay is used. Laser confocal scanning microscope is used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A -549 cells and the IC50 is respectively 3.4 ×10^-5 Stool/L, 1.8 ×10^-5mol/L and 2.6×10^-6 mol/L after treatment for 24, 48 and 72 h by juglone. Through laser confocal scanning microscope, it can be concluded that juglone can induce the apoptosis. Cell cycle changes are analyzed by flow cytometry with cells at G1 phase significantly less than those of control and cells at G2 phase significantly more than those of control. The result suggests that juglone could apoptosis of A - 549 cells with the cell cycle arrest on G2 and S phase in distinct dose-dependent manner.

关 键 词：青龙衣 胡桃醌 A-549 细胞周期 细胞凋亡 

分 类 号：R258[医药卫生—中医内科学]