紫萁组织培养的探讨  

Study on Tissue Culture of Osmunda Japonica Thunb.

作　　者：张泽宏[1] 何义发[2] 周吉源[3] 张秋萍[1] 黄剑[1] 

机构地区：[1]漳州师范学院生物科学与技术系,福建漳州363000 [2]湖北民族学院生物科学与技术学院,湖北恩施445000 [3]华中师范大学生命科学学院,湖北武汉430079

出　　处：《漳州师范学院学报（自然科学版）》2008年第4期108-112,共5页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：湖北省教育厅创新项目(2002D08001)

摘　　要：紫萁孢子叶小叶整体表面消毒比孢子粉消毒更简便易行,效果也更理想.不加任何激素与附加低浓度激素(低于1.0 mg/L)同样有很高的孢子萌发率,将MS培养基中无机盐浓度减半促进孢子萌发,糖浓度在2%-3%范围内较合适,低于2%和高出4%,孢子萌发率明显降低.25℃左右是原叶体增值的适宜温度,无机盐浓度对原叶体增殖无明显影响,培养基中附加少量活性炭和KH2PO4对原叶体增殖和生长有促进作用.6-BA0.5mg/L+NAA0.5mg/L、6-BA1.0mg/L+NAA0.5 mg/L、NAA0.5mg/L+KT 5.0mg/L和NAA1.0mg/L+KT5.0mg/L都能促进原叶体增殖.6-BA 0.5 mg/L+NAA1.0mg/L分化假根的能力最强,可作孢子体生根培养基.There was better effect on surface disinfection by using sporophyllary leaf than by using spores,and there was high germination rate of spores while the concentration of hormones were lower than 1.0 mg/L. To half the concentration of inorganic salt from MS medium was good for spores germination. The concentration of sugar was suitable from 2% to 3% or the spores germination rate would be very low. The temperature of 25℃ was suitable for prothallium proliferation. The concentration of inorganic salt could not affect obviously the prothallium proliferation. To add a little activated carbon and KH2PO4 to medium was good for prothallium proliferation.Hormones of 6-BA0.5mg/L＋NAA0.5mg/L, 6-BA1.0 mg/L＋NAA0.5mg/L, NAA0.5mg/L ＋KT 5.0mg/L, NAA1.0mg/L＋KT5.0mg/L were all good for prothallium proliferation. It was best to differentiate rhizoid by using the hormones of 6-BA 0.5mg/L＋NAA1.0mg/L.

关 键 词：紫萁 无菌培养 孢子粉 孢子叶小叶 

分 类 号：Q943.1[生物学—植物学]