两种新型倍半萜类化合物诱导人肝癌细胞SMMC-7721凋亡(英文)  

Highly oxygenated Bisabolane-type sesquiterpenes induced human hepatoma SMMC-7721 cells apoptosis

作　　者：陈瑛[1] 孟雪琴[1] 杜宇平[1] 张琪[1] 祝英[2] 王勤[1] 

机构地区：[1]兰州大学生命科学学院,甘肃兰州730000 [2]兰州大学功能有机分子化学国家重点实验室,甘肃兰州730000

出　　处：《兰州大学学报（自然科学版）》2008年第6期49-54,共6页Journal of Lanzhou University(Natural Sciences)

基　　金：Supported by the Science and Technology Support Project of Gansu Province (0708NKCA124).

摘　　要：研究两种从盘花垂头菊中分离出的高含氧甜没药烯型倍半萜(HOBS)化合物对肿瘤细胞的影响.结果显示HOBS能够诱导人肝癌细胞SMMC-7721凋亡.单细胞电泳实验证明HOBS能够以剂量依赖的方式诱导SMMC-7721细胞损伤,流式细胞技术显示HOBS能够以剂量依赖的方式诱导SMMC-7721细胞凋亡,凋亡率达到38.9%,c_i(Ca^(2+))的测定显示HOBS能够诱导c_i(Ca^(2+))升高,所有的结果证明HOBS能够诱导人肝癌细胞SMMC-7721凋亡.提示这两种化合物有抗人肝癌细胞的SMMC-7721活性,为抗肿瘤药物的设计提供了新的思路.The effects of two novel highly oxygenated bisabolane-type sesquiterpenes （HOBS） isolated from C. Discoideura （Cremanthodiurn discoidiem） on tumor cells were investigated. The results indicate that HOBS induced DNA damage and apoptosis of SMMC-7721 cells both in a dose-dependent manner as revealed by single cell gel electrophoresis and flow cytometry analyses, and the apoptosis rate was up to 38.9%. Measurement of ci（Ca^2＋） showed that HOBS could induce elevation of Ca^2＋. All the results suggest that HOBS could induce SMMC-7721 cells apoptosis.

关 键 词：肝癌细胞 SMMC-7721 凋亡 

分 类 号：Q67[生物学—生物物理学]