微乳液毛细管电动色谱法对匙鄂木中蒽醌类化合物的分离测定(英文)  

Separation and determination of anthraquinones in Paedicalyx attopevensis Pierre ex Pitard by microemulsion electrokinetic chromatography

作　　者：陈安家[1] 陈晓峰[2] 李进冉[1] 李霞[1] 李青山[1] 胡之德[3] 

机构地区：[1]山西医科大学药学院,山西太原030001 [2]兰州大学学报编辑部,甘肃兰州730000 [3]兰州大学化学化工学院,甘肃兰州730000

出　　处：《兰州大学学报（自然科学版）》2008年第6期72-77,共6页Journal of Lanzhou University(Natural Sciences)

基　　金：Supported by the Natural Science Foundation of Shanxi Province(2008011077-1);the Doctor Fondation in Shanxi Medical University(055040).

摘　　要：建立了一种快速、有效分离测定茜草科植物匙鄂木中3种蒽醌类化合物的微乳液毛细管电动色谱新方法.研究了硼砂浓度、微乳液浓度、乙腈浓度以及电压对分离行为的影响.实验结果表明:最佳缓冲体系组成为10 mmol/L硼砂-12%微乳储备液-5%乙腈,最佳运行电压为25 kV,检测波长为264 nm.分析物浓度与峰面积之间的线性关系良好,相关系数在0.999 2～0.999 5之间.该方法用于对匙鄂木中蒽醌类化合物的含量测定,回收率为97.3%～106.2%.A rapid and effective microemulsion electrokinetic chromatography method was established for simultaneous determination of three bioactive components （anthraquinones） extract of Chinese herb Paedicalyx attopevensis Pierre ex Pitard for the first time. After optimization of separation conditions, baseline separation was obtained for the three analytes within 8 min using a running buffer composed of 10 mmol/L borate solution＋12% microemulsion stock solution [sodium dodecyl sulphate （SDS）＋1- butanol＋n-heptane＋water=16.55%＋33.05%＋4.05%＋46.35% （w/w）]＋5% acetonitrile. Regression equations revealed linear relationships （correlation coefficients 0.999 2-0.999 5） between the peak area of each analyte and the concentration. The relative standard deviations of the migration times and the peak areas of the three constituents were less than 0.53% and 3.2%. The recoveries of three constituents ranged from 97.3% to 106.2%.

关 键 词：匙鄂木 蒽醌 微乳液毛细管电动色谱 

分 类 号：O657.8[理学—分析化学]