职业应激易感性与肿瘤坏死因子-α基因多态性的关系  

Association of tumor necrosis factor-α gene polymorphisms with susceptibility to occupational stress

作　　者：邱英[1,2] 余善法[2] 

机构地区：[1]郑州大学公共卫生学院,450052 [2]河南省职业病防治研究所

出　　处：《中华劳动卫生职业病杂志》2008年第12期715-717,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的研究肿瘤坏死因子-α（TNF-α）基因多态性与职业应激易感性的关系。方法应用职业紧张测量工具对169名流水线作业工人进行职业应激测量，依据职业应激评分将其分为高、低应激组，采用聚合酶链反应-限制性片段长度多态性分析其TNF—α基因多态位点的变化。结果低应激组中TNF-α-308位点G／G基因型34例、G／A基因型4例，而高应激组中G／G基因型117例、G／A基因型10例、A从基因型1例；低应激组中TNF-α-308位点A的分布频率与高应激组差异无统计学意义（校正X^2=0．0001，P〉0．05）。在低应激组中TNF-α-238位点G／G基因型30例、G／A基因型7例、刖A基因型1例，而高应激组中G／G基因型118例、G／A基因型10例，低应激组中TNF—α-238位点等位基因A的分布频率高于高应激组，差异有统计学意义（校正X^2=5．449，P〈0．05）。结论TNF—α-238位点多态性可能与职业应激易感性有关，而TNF-α-308位点多态性可能与职业应激遗传易感性无明显关联。Objeαive To find out whether tumor necrosis faαor （TNF）-α-308 and TNF-α-238 locus （G→A） mutation within the TNF-α gene promoter region is associated with susceptibility to occupational stress. Methods 169 pipelining workers were investigated using the occupational stress measuring instru- ment. The subjeαs were divided into high and low strain groups according to the job strain score of the questionnaire. By means of polymerase chain reaαion-restriαion fragment length polymorphisms （PCR-RFLP） techniques, TNF-α gene polymorphisms of all subjeαs were analyzed. Results There were 34 workers with genotype -308G/G and 4 with -308G/A genotype in the low strain group while in the high strain group, there were 117 workers with genotype -308G/G and 10 with -308G/A genotype and 1 with -308A/A. No significant difference in -308 genotype was found between the two groups （X^2=0.0001 ,P〉0.05 ）. There were 30 workers with genotype -238G/G and 7 with -238G/A genotype and 1 with -238A/A in the low strain group while there were 118 workers with genotype -238G/G and 10 with -238G/A genotype in high strain group. The frequencies of TNF-α-238A in the low strain groups were significantly higher than that in high strain groups （X^2 =5.45 ,P〈0.05）. Conclusion TNF-α promoter G238A polymorphism is associated with occupational stress. There is no evident relationship between TNF-α promoter G308A polymorphism and susceptibility to occupational stress.

关 键 词：应激 多态性 单核苷酸 肿瘤坏死因子 

分 类 号：R686[医药卫生—骨科学]